The carbon dots with near-infrared electrochemiluminescence emission and low excitation potential for ultrasensitive analysis of microRNA-222
作者全名：Guo, Yu-Zhuo; Liu, Rui; Zeng, Ying; Liao, Ye-Yu; Liu, Jia-Li; Chai, Ya-Qin; Yuan, Ruo
作者地址：[Guo, Yu-Zhuo; Zeng, Ying; Liao, Ye-Yu; Liu, Jia-Li; Chai, Ya-Qin; Yuan, Ruo] Southwest Univ, Minist Educ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing Southwest, Chongqing 400715, Peoples R China; [Liu, Rui] Chongqing Med Univ, Dept Oncol, Affiliated Hosp 2, Chongqing 400000, Peoples R China
通信作者：Liu, JL; Chai, YQ; Yuan, R (通讯作者)，Southwest Univ, Minist Educ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing Southwest, Chongqing 400715, Peoples R China.; Liu, R (通讯作者)，Chongqing Med Univ, Dept Oncol, Affiliated Hosp 2, Chongqing 400000, Peoples R China.
来源：CHEMICAL ENGINEERING JOURNAL
ESI学科分类：ENGINEERING
WOS号：WOS:001228808500001
JCR分区：Q1
影响因子：13.3
年份：2024
卷号：488
期号：
开始页：
结束页：
文献类型：Article
关键词：Carbon dots; Near -infrared electrochemiluminescence; Biosensor; MicroRNA
摘要：In this work, nitrogen and sulfur codoped near -infrared emission carbon dots (N,S-NIR-CDs) were used as neoteric electrochemiluminescence (ECL) emitters with high efficiency to construct a biosensor for ultrasensitive analysis of microRNA-222 (miRNA-222), which has been regarded as prospective biomarkers for early cancer diagnosis. Impressively, compared to conventional CDs with short ECL wavelength and large excitation voltage, N,S-NIR-CDs possessed wide sp 2 domains and narrow band gap, leading to the near -infrared ECL emission at 680 nm and low excitation potential from -1.0 to 0 V, which not only decreased the photochemical damage for biomolecules but also reduced the side reaction. Moreover, an improved T7 Exonuclease induced cascade amplification (EICA) strategy was designed to transform trace miRNA-222 into plentiful output DNA in dualcycle for greatly improving the target conversion efficiency. Therefore, by combining the N,S-NIR-CDs as neoteric ECL emitters and EICA strategy as signal amplifier, the constructed ECL biosensor could realize the ultrasensitive analysis of miRNA-222 with a identification limit of 5.65 aM and successfully employed in miRNA222 analysis from liver cancer cell (HCCLM3 and MHCC-97L) lysate. This strategy developed a kind of neoteric CDs with NIR ECL emission and low excitation potential to establish a highly efficient biosensor for biomarker analysis, which prepared a road for early diseases diagnosis and lied a foundation for biological ECL imaging.
基金机构：National Natural Science Foundation of China [22176153, 22374122, 22174113, 22304141]; Chongqing Postdoctoral Innovative Talent Support Program [7820100788]; Chongqing Postdoctoral Natural Science Foundation [CSTB2023NSCQ-BHX0009]
基金资助正文：This work was financially supported by the National Natural Science Foundation of China (22176153, 22374122, 22174113 and 22304141) , Chongqing Postdoctoral Innovative Talent Support Program (7820100788) and Chongqing Postdoctoral Natural Science Foundation (CSTB2023NSCQ-BHX0009) .
