CircMYBL1 suppressed acquired resistance to osimertinib in non-small-cell lung cancer

作者全名：Li, Yaji; Wang, Nan; Huang, Yutang; He, Shuai; Bao, Meihua; Wen, Chunjie; Wu, Lanxiang

作者地址：[Li, Yaji; Wang, Nan; Huang, Yutang; He, Shuai; Wen, Chunjie; Wu, Lanxiang] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [He, Shuai] Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China; [Bao, Meihua] Changsha Med Univ, Hunan Key Lab Res & Dev Novel Pharmaceut Preparat, Changsha 410219, Peoples R China; [Bao, Meihua] Changsha Med Univ, Academician Workstn, Changsha 410219, Peoples R China

通信作者：Wen, CJ; Wu, LX (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China.

来源：CANCER GENETICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001230406600001

JCR分区：Q4

影响因子：1.4

年份：2024

卷号：284-285

期号：　

开始页：34

结束页：42

文献类型：Article

关键词：circRNA; Osimertinib; Acquired resistance; Non -small -cell lung cancer; RNA sequencing

摘要：Circular RNAs (circRNAs) play an important role in the development of acquired resistance to many anticancer drugs. We developed the Non -Small -Cell Lung Cancer (NSCLC) cell lines with acquired resistance to osimertinib, a third -generation of epidermal growth factor receptor -tyrosine kinase inhibitors (EGFR-TKIs), and evaluated the different expression profiles of circRNAs in osimertinib-sensitive and -resistant NSCLC cell lines using RNA sequencing (RNA-Seq). The expression of selected differentially expressed circRNAs was verified using quantitative real-time PCR (qRT-PCR) in paired osimertinib-sensitive and -resistant NSCLC cell lines, and in plasma samples of osimertinib-sensitive and -resistant NSCLC patients. We found that circMYBL1(has_circ_0136924) was downregulated after acquired resistance to osimertinib, inhibiting circMYBL1 expression facilitated the proliferation, migration, and invasion in osimertinib-sensitive NSCLC cells. CircMYBL1 may be a novel molecular biomarker and therapeutic target for osimertinib-resistant NSCLC.

基金机构：Natural Science Foundation of China [82073938, 82274023, 82373135]; Youth Innovation in Future Medicine, Chongqing Medical University [W0093]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN202200432]

基金资助正文：This work was supported by the Natural Science Foundation of China (No. 82073938, 82274023, 82373135) , Youth Innovation in Future Medicine, Chongqing Medical University (No. W0093) , and the Scientific and Technological Research Program of Chongqing Municipal Education Commission (No. KJQN202200432) .