Total duration of spontaneous blastocyst collapse during the expansion stage is an independent predictor of euploidy and live birth rates

作者全名：Zhu, Jiahong; Zou, Jiayi; Wu, Lihong; Xiong, Shun; Gao, Yang; Liu, Junxia; Huang, Guoning; Han, Wei

作者地址：[Zhu, Jiahong; Zou, Jiayi; Wu, Lihong; Xiong, Shun; Gao, Yang; Liu, Junxia; Huang, Guoning; Han, Wei] Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China; [Zhu, Jiahong; Zou, Jiayi; Wu, Lihong; Xiong, Shun; Gao, Yang; Liu, Junxia; Huang, Guoning; Han, Wei] Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China

通信作者：Huang, GN; Han, W (通讯作者)，Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China.; Huang, GN; Han, W (通讯作者)，Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China.

来源：REPRODUCTIVE BIOMEDICINE ONLINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001232049600001

JCR分区：Q1

影响因子：3.7

年份：2024

卷号：49

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Number of collapses; Total duration of collapse to re-expansion; Euploidy; Embryo quality

摘要：Research question: Is the total duration of spontaneous blastocyst collapse to re -expansion before biopsy related to ploidy and live birth rates after single euploid blastocyst transfer? Design: This was a retrospective cohort study of 600 preimplantation genetic testing cycles for aneuploidy (PGT-A) cycles, involving 2203 biopsied blastocysts, at a large reproductive medicine centre. Features of spontaneous blastocyst collapse from full to expanded stage, before biopsy, were observed using an embryoscope viewer for embryos cultured in a time-lapse incubator. In total, 568 cycles of frozen blastocyst transfers, either single euploid or mosaic, were performed. Correlations between collapse features and PGT-A outcomes were evaluated, as well as live birth rate, following euploid embryo transfer. Results: Blastocysts with lower morphological quality or delayed development had signi fi cantly higher rates of collapse, multiple collapses, and a longer duration of collapse to re -expansion. After controlling for confounders, such as oocyte age, morphological quality of blastocyst, and day of biopsy, multivariate logistic regression revealed that the total duration of collapse to re -expansion was an independent predictor of lower euploidy rate; the multivariate OR was 0.85 (95% CI 0.77-0.95; P = 0.00). Furthermore, even with euploid embryo transfer, the probability of a live birth decreased as the total duration of collapse to re -expansion increased; the multivariate OR was 0.79 (95% CI 0.64-0.98; P = 0.033). Conclusion: The total duration of blastocyst collapse to re -expansion could be used as a predictor of lower euploidy and live birth rate. When developing blastocyst algorithms for pregnancy prediction, the duration of spontaneous blastocyst collapse should be included as a signi fi cant variable.

基金机构：Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission [CSTB2023NSCQ-MSX0258, CSTB2022NSCQ-MSX0253]

基金资助正文：This work was supported by the Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (Grant Nos. CSTB2023NSCQ-MSX0258; and CSTB2022NSCQ-MSX0253) .