Ciprofol ameliorates ECS-induced learning and memory impairment by modulating aerobic glycolysis in the hippocampus of depressive-like rats

作者全名：Yang, You; Zhou, Dongyu; Min, Su; Liu, Di; Zou, Mou; Yu, Chang; Chen, Lihao; Huang, Jia; Hong, Ruiyang

作者地址：[Yang, You; Zhou, Dongyu; Min, Su; Zou, Mou; Yu, Chang; Chen, Lihao; Huang, Jia; Hong, Ruiyang] Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Liu, Di] First Peoples Hosp Neijiang, Dept Anesthesiol, Neijiang, Sichuan, Peoples R China

通信作者：Min, S (通讯作者)，Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China.

来源：PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001235090200001

JCR分区：Q1

影响因子：3.3

年份：2024

卷号：239

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Ciprofol; Depression; Electroconvulsive shock; Aerobic glycolysis; Propofol

摘要：Electroconvulsive shock (ECS) is utilized to treat depression but may cause learning/memory impairments, which may be ameliorated by anesthetics through the modulation of hippocampal synaptic plasticity. Given that synaptic plasticity is governed by aerobic glycolysis, it remains unclear whether anesthetics modulate aerobic glycolysis to enhance learning and memory function. Depression-like behavior in rats was induced by chronic mild unpredictable stress (CUMS), with anhedonia assessed via sucrose preference test (SPT). Depressive-like behaviors and spatial learning/memory were assessed with forced swim test (FST), open field test (OFT), and Morris water maze (MWM) test. Changes in aerobic glycolysis and synaptic plasticity in the hippocampal region of depressive-like rats post-ECS were documented using immunofluorescence analysis, Western blot, Lactate Assay Kit and transmission electron microscopy. Both the OFT and FST indicated that ECS was effective in alleviating depressive-like behaviors. The MWM test demonstrated that anesthetics were capable of attenuating ECS-induced learning and memory deficits. Immunofluorescence analysis, Western blot, Lactate Assay Kit and transmission electron microscopy revealed that the decline in learning and memory abilities in ECS-induced depressive-like rats was correlated with decreased aerobic glycolysis, and that the additional use of ciprofol or propofol ameliorated these alterations. Adding the glycolysis inhibitor 2-DG diminished the ameliorative effects of the anesthetic. No significant difference was observed between ciprofol and propofol in enhancing aerobic glycolysis in astrocytes and synaptic plasticity after ECS. These findings may contribute to understanding the mechanisms by which anesthetic drugs modulate learning and memory impairment after ECS in depressive-like behavior rats.

基金机构：National Natural Science Foundation of China [81,873,798, 81,901,377]; National Key Clinical Specialty Construction Project of China [2011-170]; Chongqing Medical Key Discipline Construction Project [2007-2]; Foundation of Postgraduate Innovation Project of Chongqing [CYB20144]; Chongqing Science and Technology Bureau [cstc2019jcyj-msxmX0839]; Sichuan Medical Association Youth Research Innovation Fund Project [Q22022]

基金资助正文：This work is funded by the National Natural Science Foundation of China (No. 81,873,798 and No. 81,901,377) , the National Key Clinical Specialty Construction Project of China (No. 2011-170) , the Chongqing Medical Key Discipline Construction Project (No. 2007-2) , the Foundation of Postgraduate Innovation Project of Chongqing (CYB20144) , Chongqing Science and Technology Bureau under Grant (No cstc2019jcyj-msxmX0839) , and the Sichuan Medical Association Youth Research Innovation Fund Project (No. Q22022) . The funders had no role in study design, data collection, analysis, interpretation, or the decision to submit for publication. The views expressed are those of the author (s) and not necessarily those of the funders.