Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

作者全名：Zhang, Hong; Zou, Heng; Zhang, Chanyu; Zhang, Shen

作者地址：[Zhang, Hong; Zou, Heng; Zhang, Chanyu; Zhang, Shen] Chongqing Med Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Zhang, Chanyu; Zhang, Shen] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Minist Educ China, Chongqing 400010, Peoples R China

通信作者：Zhang, S (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Affiliated Hosp 2, Chongqing 400010, Peoples R China.; Zhang, S (通讯作者)，Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Minist Educ China, Chongqing 400010, Peoples R China.

来源：ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001235601900001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Chronic endometritis; Endometrial microbiota; Recurrent implantation failure; Reproductive outcomes; Microbial diversity

摘要：Background Chronic endometritis (CE) is associated with poor reproductive outcomes, yet the role of endometrial microbiota in patients with recurrent implantation failure (RIF) and CE remains unclear. This study aims to characterize endometrial microbiota in RIF patients with CE and assess its implications for reproductive outcomes.Methods In this prospective study, we enrolled RIF patients both with and without CE. Endometrial and cervical samples were collected for 16 S rRNA gene sequencing. Microbiota composition was compared between groups using diversity indices, phylum, and genus-level analysis. Canonical correlation analysis (CCA) and Spearman's correlation coefficients were used to assess relationships between CE, reproductive outcomes, and microbiota. Predictive functional profiling was performed to evaluate metabolic pathways associated with CE.Results Endometrial microbiota in CE patients exhibited greater diversity and evenness compared to non-CE patients. Principal coordinates analysis (PCoA) revealed distinct clustering between CE and non-CE groups. Linear discriminant analysis (LDA) identified Proteobacteria, Aminicenantales, and Chloroflexaceae as characteristic of CE, while Lactobacillus, Acinetobacter, Herbaspirillum, Ralstonia, Shewanela, and Micrococcaceae were associated with non-CE. CCA demonstrated associations between CE, adverse reproductive outcomes, and specific bacterial taxa. Microbial metabolic pathways significantly differed between CE and non-CE groups, with enrichment in pathways related to cofactors, vitamins, secondary metabolites, and the immune system in CE patients.Conclusion RIF patients with CE exhibit distinct endometrial microbiota compositions associated with adverse reproductive outcomes. The increased microbial diversity and altered metabolic pathways in CE suggest a potential correlation with reproductive outcomes, although further studies are necessary to elucidate the causal relationship between microbiota alterations and fertility. Modulating the endometrial microbiome may represent a novel therapeutic strategy to improve IVF outcomes in patients with CE.

基金机构：National Natural Science Foundation of China

基金资助正文：Not applicable.