Facile fabrication of anticancer peptide coated zeolite imidazole framework for effective treatment of colorectal cancer cells and its apoptosis induction

作者全名：Wang, Chun-Yi; Li, Dairong; Chen, Xiufeng; Chen, Zhixiong

作者地址：[Wang, Chun-Yi] Chongqing Med Univ, Dept Gastrointestinal Surg, Affiliated Hosp 1, Chongqing, Peoples R China; [Li, Dairong] Chongqing Univ, Dept Med Oncol, Canc Hosp, Chongqing 400030, Peoples R China; [Chen, Xiufeng; Chen, Zhixiong] Chongqing Univ, Gastrointestinal Canc Ctr, Canc Hosp, Chongqing 400030, Peoples R China

通信作者：Chen, XF; Chen, ZX (通讯作者)，Chongqing Univ, Gastrointestinal Canc Ctr, Canc Hosp, Chongqing 400030, Peoples R China.

来源：PROCESS BIOCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001236559900001

JCR分区：Q2

影响因子：3.7

年份：2024

卷号：143

期号：　

开始页：34

结束页：43

文献类型：Article

关键词：Anticancer peptides; Colorectal cancer; Drug delivery; Nanoparticles; ZIF-8

摘要：The low drug resistance, excellent cancer cell targeting, and anticancer benefits of anticancer peptides (ACPs) make them an attractive antitumor drug option. On the other hand, enzymes readily break down in many ACPs after delivery due to their non-specific toxicity. Consequently, drug delivery systems (DDSs) are necessary to avoid the peptides ' dissociation and persuade targeted delivery. The cationic amphiphilic anticancer peptide, G (IIKK) 3 I-NH 2 (G3), was developed in this work using a high -performance microfluidic system, which encapsulates the zeolitic imidazolate framework (ZIF-8). The ACPs-loaded nanoparticles (NPs) were efficiently and quickly mixed using the microfluidic system, resulting in NPs with tunable properties. The encapsulation efficacy was high, and the production rate was 120 mL/min. The microfluidic technique-based ZIF-8 fabrication demonstrated better size homogeneity, as evidenced by a decreased polydispersity index (PDI) compared to the conventional technique. The hemolytic effect was much diminished, and a pH -controlled release of the G3 peptides was achieved by encapsulating G(IIKK) 3 I-NH 2 (termed as G3@ZIF-8) into the ZIF-8. Owing to the improved cell absorption by the ZIF-8, G3@ZIF-8 demonstrated a stronger anticancer impact than the free G3 peptide at identical concentrations. In addition to damaging the mitochondrial membrane of HCT 116 colorectal cancer cells, the NPs were able to suppress the proliferation. Consequently, ACP-based cancer therapy can be expanded by developing G3-loaded ZIF-8-NPs, offering an option technique for ACP-based delivery.

基金机构：Chongqing Science and Health Joint Project [2021MSXM096]

基金资助正文：This work was supported by the Chongqing Science and Health Joint Project (Project number: 2021MSXM096) .