Fenton reaction enhanced enzyme free electrochemical biosensor for rapid and highly sensitive detection of Salmonella

作者全名：Zheng, Ying; Li, Qing; Yang, Zhongzhu; Yang, Ruyi; Liu, Xin; Ding, Shijia; Han, Daobin

作者地址：[Zheng, Ying] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Dept Lab Med, Chengdu, Peoples R China; [Zheng, Ying] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu, Peoples R China; [Li, Qing] Third Peoples Hosp Jinan, Jinan 250000, Shandong, Peoples R China; [Yang, Zhongzhu; Yang, Ruyi; Liu, Xin] Chengdu Univ Tradit Chinese Med, Coll Med Technol, Chengdu 611137, Peoples R China; [Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Han, Daobin] Shandong Univ, Hosp 2, Dept Clin Lab, Jinan 250000, Shandong, Peoples R China

通信作者：Han, DB (通讯作者)，Shandong Univ, Hosp 2, Dept Clin Lab, Jinan 250000, Shandong, Peoples R China.

来源：JOURNAL OF ELECTROANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001236627000001

JCR分区：Q1

影响因子：4.1

年份：2024

卷号：962

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Fenton reaction; ConA; Electrochemical biosensor; Salmonella

摘要：To fulfill the stringent demands of clinical Salmonella detection, this study employed the benefits of Ni-TCPP (Fe2+) and ConA to establish a novel approach that is culture-free, enzyme-free, secondary antibody-free, and ultra-sensitive. Our investigations revealed two key mechanisms that significantly bolster the signal-to-noise ratio (SNR) and enhance detection stability: firstly, the Fenton reaction and the mimic enzyme catalysis of NiTCPP(Fe2+) improved the sensitivity of the biosensor; secondly, the substitution of the secondary antibody with ConA led to increased binding of Ni-TCPP(Fe2+) due to the abundance of ConA receptors on the bacterial surface. With proper assembly, the developed biosensor exhibited high sensitivity in the range of 101-107 CFU/mL, along with a detection limit as low as 4 CFU/mL, and achieved rapid detection within a mere 2 h. Moreover, this biosensor has proven its efficacy in detecting Salmonella in clinical biological samples. Its advantages of being culture-free, secondary antibody-free, and ultra-sensitive establish a solid research foundation for the expedited detection of Salmonella in clinical settings and point-of-care testing (POCT).

基金机构：National Natural Science Foundation of China [81371904]; Natural Science Youth Foundation of Shan- dong Province [ZR2021QH055]

基金资助正文：This work was funded by the National Natural Science Foundation of China (81371904) and the Natural Science Youth Foundation of Shan- dong Province (ZR2021QH055) .