AND Logic-Gate-Based Dual-Locking Probe System for the Sensitive Detection of microRNA and APE1

作者全名：Wang, Jiani; Han, Xiaole; Yu, Hongyan; Xie, Guoming

作者地址：[Wang, Jiani; Han, Xiaole; Yu, Hongyan; Xie, Guoming] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Wang, Jiani] Sichuan Nursing Vocat Coll, Chengdu 610100, Peoples R China

通信作者：Xie, GM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chinese Minist Educ, Chongqing 400016, Peoples R China.

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001237233200001

JCR分区：Q1

影响因子：6.7

年份：2024

卷号：96

期号：23

开始页：9570

结束页：9575

文献类型：Article

关键词：　

摘要：MicroRNA (miRNA) and apurinic/apyrimidinic endonuclease 1 (APE1) have been reported to be closely associated with cancers, making them potential crucial biomarkers and therapeutic targets. However, focusing on the detection of a single target is not conducive to the diagnosis and prognosis assessment of diseases. In this study, an AND logic-gate-based dual-locking hairpin-mediated catalytic hairpin assembly (DL-CHA) was developed for sensitive and specific detection of microRNA and APE1. By addition of a lock to each of the hairpins, with APE1 and microRNA serving as keys, fluorescence signals could only be detected in the presence of simultaneous stimulation by APE1 and miRNA-224. This indicated that the biosensor could operate as an AND logic gate. DL-CHA exhibited advantages such as a low background, rapid response, and high logic capability. Therefore, the biosensor serves as a novel approach to cancer diagnosis with significant potential applications.

基金机构：National Natural Science Foundation of China [2022YFC2603800]; National Key R&D Program of China [82172369]; National Natural Science Foundation of China

基金资助正文：This work was supported by the National Key R&D Program of China (2022YFC2603800) and the National Natural Science Foundation of China (82172369).