New electrocatalyst of Ni₆MnO₈@AB/PtNPs with efficient synergistic enhancement in electrochemical aptasensor for sensitive detection of neuron specific enolase

作者全名：Wu, Liping; Qing, Min; Mu, Zhaode; Zhou, Jing; Zuo, Jianli; Bai, Lijuan

作者地址：[Wu, Liping; Qing, Min; Mu, Zhaode; Zhou, Jing; Zuo, Jianli; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China

通信作者：Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China.

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:001238002600001

JCR分区：Q1

影响因子：4.9

年份：2024

卷号：201

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Neuron -specific enolase; Electrochemical aptasensor; Ternary transition metal oxide; Acetylene black; Platinum nanoparticles; Synergistic catalysis

摘要：As one of the specific markers for small cell lung cancer (SCLC), the clinical value for serum neuron-specific enolase (NSE) detection in diagnosis, monitoring and therapy of SCLC is considerable. Herein, a sensitive amperometric aptasensor was developed for the detection of NSE based on nickel-manganese oxide@acetylene black/platinum nanoparticles (Ni6MnO8@AB/PtNPs) composite as signal amplifier. The combination of Ni6MnO8, AB and PtNPs not only showed synergistically catalytic activity to hydrogen peroxide (H2O2), but also possessed lots of binding sites for loading the thiol-terminated signal probes (TSPs) through Pt-S bonds. Additionally, nitrogen-doped carbon nanotubes/poly (diallyldimethylammonium chloride)/ZnO microflowers (N-CNTs/P/ZnO MFs) exhibited outstanding electric conductivity and was used as substrate material to load more biotin-terminated capture probes (BCPs). In the presence of the NSE, the TSPs and BCPs specifically recognized the NSE to form a sandwich-type electrochemical aptasensor, which exhibited a broad linear response range from 0.00001 similar to 100 ng mL(-1) and a low detection limit of 2.86 fg mL(-1) (S/N = 3). In addition, the electrochemical aptasensor obtained favorable detection results in diluted human serum, indicating that it had potential application in clinical NSE detection.

基金机构：National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Special Funding for Post- doctoral Research Project of Chongqing, China [2021XM2045]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-BHX0006]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Top Talent Cultivation Plan for Postgraduate of Chongqing Medical University, China [BJRC202127]

基金资助正文：This work is supported by National Natural Science Foundation of China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Special Funding for Post- doctoral Research Project of Chongqing, China (2021XM2045) , Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006) , Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Top Talent Cultivation Plan for Postgraduate of Chongqing Medical University, China (BJRC202127) .