Effect of<i> in</i><i> vivo</i> culture conditions on the proliferation and differentiation of rat adipose-derived stromal cells

作者全名：Wang, Chao; Xie, Tian; Li, Xiaoming; Lu, Xue; Xiao, Changxue; Liu, Ping; Xu, Feng; Zhang, Bo

作者地址：[Wang, Chao; Lu, Xue; Xiao, Changxue; Xu, Feng] Chongqing Med Univ, Dept Intens Care Unit, Childrens Hosp, Chongqing 400014, Peoples R China; [Wang, Chao; Lu, Xue; Xiao, Changxue; Xu, Feng] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders, Chongqing 400016, Peoples R China; [Wang, Chao; Lu, Xue; Xiao, Changxue; Xu, Feng] Chongqing Key Lab Pediat Metab & Inflammat Dis, Chongqing 400016, Peoples R China; [Xie, Tian] Army Med Univ, Daping Hosp, Dept Radiol, Chongqing 400042, Peoples R China; [Li, Xiaoming; Liu, Ping] Army Med Univ, Daping Hosp, Dept Mil Traff Injury Prevent, Chongqing 400042, Peoples R China; [Zhang, Bo] Army Med Univ, Daping Hosp, Inst Surg Res, State Key Lab Trauma Burns & Combined Injury, Chongqing 400042, Peoples R China

通信作者：Xu, F (通讯作者)，Chongqing Med Univ, Dept Intens Care Unit, Childrens Hosp, Chongqing 400014, Peoples R China.; Zhang, B (通讯作者)，Army Med Univ, Daping Hosp, Inst Surg Res, State Key Lab Trauma Burns & Combined Injury, Chongqing 400042, Peoples R China.

来源：MECHANISMS OF AGEING AND DEVELOPMENT

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001238041600001

JCR分区：Q1

影响因子：5.3

年份：2024

卷号：219

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Adipose -derived stromal cells (ADSCs); Proliferation; Differentiation; Multipotency; RNA sequencing

摘要：Adipose -derived stromal cells (ADSCs) are promising stem cell sources for tissue engineering and cell -based therapy. However, long-term in vitro expansion of ADSCs impedes stemness maintenance, which is partly attributed to deprivation of their original microenvironment. Incompetent cells limit the therapeutic effects of ADSC-based clinical strategies. Therefore, reconstructing a more physiologically and physically relevant niche is an ideal strategy to address this issue and therefore facilitates the extensive application of ADSCs. Here, we transplanted separated ADSCs into local subcutaneous adipose tissues of nude mice as an in vivo cell culture model. We found that transplanted ADSCs maintained their primitive morphology and showed improved proliferation and delayed senescence compared to those of cells cultured in an incubator. Significantly increased expression of stemness-related markers and multilineage differentiation abilities were further observed in in vivo cultured ADSCs. Finally, sequencing revealed that genes whose expression differed between ADSCs obtained under in vivo and in vitro conditions were mainly located in the extracellular matrix and extracellular space and that these genes participate in regulating transcription and protein synthesis. Moreover, we found that an Egr1 signaling pathway might exert a crucial impact on controlling stemness properties. Our findings might collectively pave the way for ADSC-based applications.

基金机构：Youth Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders [YBRP-202118]

基金资助正文：This work was financially supported by the Youth Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders (No. YBRP-202118) .