Accessing Early Differentiation of Virus-Specific Follicular Helper CD4 <SUP>+</SUP> T Cell in Acute LCMV-Infected Mice

作者全名：Lin, Yao; Yue, Shuai; Yang, Yang; He, Junjian; Yang, Xiaofan; Ye, Lilin; Chen, Xiangyu

作者地址：[Lin, Yao] Shenzhen Univ, South China Hosp, Med Sch, Dept Urol, Shenzhen, Peoples R China; [Lin, Yao] Shenzhen Univ, Sch Biomed Engn, Guangdong Key Lab Biomed Measurements & Ultrasoun, Natl Reg Key Technol Engn Lab Med Ultrasound,Med, Shenzhen, Peoples R China; [Yue, Shuai] Third Mil Med Univ, Daping Hosp, Canc Ctr, Chongqing, Peoples R China; [Yue, Shuai] Third Mil Med Univ, Army Med Ctr PLA, Chongqing, Peoples R China; [Yang, Yang] Southern Med Univ, Sch Lab Med & Biotechnol, Guangdong Prov Key Lab Immune Regulat & Immunothe, Guangzhou, Peoples R China; [He, Junjian; Ye, Lilin] Third Mil Med Univ, Inst Immunol, Chongqing, Peoples R China; [Yang, Xiaofan] Southern Med Univ, Dermatol Hosp, Guangzhou, Peoples R China; [Chen, Xiangyu] Chongqing Med Univ, Inst Immunol Innovat & Translat, Chongqing, Peoples R China

通信作者：Ye, LL (通讯作者)，Third Mil Med Univ, Inst Immunol, Chongqing, Peoples R China.; Chen, XY (通讯作者)，Chongqing Med Univ, Inst Immunol Innovat & Translat, Chongqing, Peoples R China.

来源：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001238338000022

JCR分区：Q3

影响因子：1.2

年份：2024

卷号：　

期号：206

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要：Follicular Helper T (T-FH) cells are perceived as an independent CD4 (+) T cell lineage that assists cognate B cells in producing high-affinity antibodies, thus establishing long-term humoral immunity. During acute viral infection, the fate commitment of virus-specific T-FH cells is determined in the early infection phase, and investigations of the early-differentiated T-FH cells are crucial in understanding T cell-dependent humoral immunity and optimizing vaccine design. In the study, using a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection and the TCR-transgenic SMARTA (SM) mouse with CD4 (+) T cells specifically recognizing LCMV glycoprotein epitope I-A(b)GP 66-77 , we described procedures to access the early fate commitment of virus-specific T-FH cells based on flow cytometry stainings. Furthermore, by exploiting retroviral transduction of SM CD4 (+) T cells, methods to manipulate gene expression in early-differentiated virus-specific T-FH cells are also provided. Hence, these methods will help in studies exploring the mechanism(s) underlying the early commitment of virus-specific T-FH cells.

基金机构：National Natural Science Foundation of China [32300785]; China National Postdoctoral Program for Innovative Talents [BX20230449]; National Science and Technology Major Project [2021YFC2300602]

基金资助正文：This study was supported by grants from the National Natural Science Foundation of China (No. 32300785 to X.C.) , the China National Postdoctoral Program for Innovative Talents (No. BX20230449 to X.C.) , and the National Science and Technology Major Project (No. 2021YFC2300602 to L.Y.) .