Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma

作者全名：Yang, Jia; Zhong, Xiaoni; Gao, Xiaoling; Xie, Wenyi; Chen, Yaokai; Liao, Yuanjiang; Zhang, Peilin

作者地址：[Yang, Jia; Zhong, Xiaoni] Chongqing Med Univ, Coll Publ Hlth, Chongqing, Peoples R China; [Yang, Jia; Gao, Xiaoling; Xie, Wenyi; Liao, Yuanjiang] Chongqing Ninth Peoples Hosp, Dept Nephrol, Chongqing, Peoples R China; [Yang, Jia; Xie, Wenyi; Zhang, Peilin] Chongqing Ninth Peoples Hosp, Cost Management Res Ctr, Chongqing, Peoples R China; [Chen, Yaokai] Chongqing Publ Hlth Med Ctr, Sci Res & Educ Dept, Chongqing, Peoples R China; [Zhang, Peilin] Chongqing Ninth Peoples Hosp, Cost Management Res Ctr, 69 Jialing Village, Beibei 400700, Chongqing, Peoples R China

通信作者：Zhang, PL (通讯作者)，Chongqing Ninth Peoples Hosp, Cost Management Res Ctr, 69 Jialing Village, Beibei 400700, Chongqing, Peoples R China.

来源：CELL ADHESION & MIGRATION

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001239060000001

JCR分区：Q3

影响因子：3.3

年份：2024

卷号：18

期号：1

开始页：1

结束页：12

文献类型：Article

关键词：Migration; proliferation; renal cell carcinoma; tumor growth

摘要：In this research, we investigated the role of PIK3R6, a regulatory subunit of PI3K gamma, known for its tumor-promoting properties, in clear cell renal cell carcinoma (CCRCC). Utilizing the UALCAN website, we found PIK3R6 upregulated in CCRCC, correlating with lower survival rates. We compared PIK3R6 expression in CCRCC tumor tissues and adjacent normal tissues using immunohistochemistry. Post RNA interference-induced knockdown of PIK3R6 in 786-O and ACHN cell lines, we performed CCK-8, colony formation, Edu staining, flow cytometry, wound healing, and transwell assays. Results showed that PIK3R6 silencing reduced cell proliferation, migration, and invasion, and induced G0/G1 phase arrest and apoptosis. Molecular analysis revealed decreased CDK4, Cyclin D1, N-cadherin, Vimentin, Bcl-2, p-PI3K and p-AKT, with increased cleaved caspase-3, Bax, and E-cadherin levels in CCRCC cells. Moreover, inhibiting PIK3R6 hindered tumor growth. These findings suggest a significant role for PIK3R6 in CCRCC cell proliferation and metastasis, presenting it as a potential therapeutic target.

基金机构：Chongqing Municipal Health Commission and Science and Technology Bureau Joint Medical Research Project [2022ZDXM024]

基金资助正文：This work is supported by the Chongqing Municipal Health Commission and Science and Technology Bureau Joint Medical Research Project [NO. 2022ZDXM024].