A ratiometric fluorescent probe with dual-targeting capability for heat shock imaging
作者全名：Cao, Ting; Xu, Zhongsheng; Dong, Wenhua; Ma, Hong; Fan, Zhefeng; Liu, Yun
作者地址：[Cao, Ting; Dong, Wenhua; Fan, Zhefeng] Shanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Magnet Mol & Magnet Informat Mat, Minist Educ, Taiyuan 030031, Peoples R China; [Xu, Zhongsheng; Liu, Yun] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Ma, Hong] Taiyuan Normal Univ, Coll Chem & Mat, Jinzhong 030619, Peoples R China
通信作者：Fan, ZF (通讯作者)，Shanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Magnet Mol & Magnet Informat Mat, Minist Educ, Taiyuan 030031, Peoples R China.; Liu, Y (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China.
来源：TALANTA
ESI学科分类：CHEMISTRY
WOS号：WOS:001239185500001
JCR分区：Q1
影响因子：5.6
年份：2024
卷号：276
期号：
开始页：
结束页：
文献类型：Article
关键词：
摘要：HSO3- is an important reactive sulfur species that maintains the normal physiological activities of living organisms and participates in a variety of redox homeostatic processes. It has been found that changes in HSO3- levels is closely related to the heat stroke phenomenon of the organism. Heat stroke causes damage to normal cells, which in turn causes damage to the body and even death. It is crucial to accurately monitor and track the physiological behavior of HSO3- during heat stroke. Herein, a ratiometric multifunctional fluorescent probe DRM-SO2 with dual-targeting ability to rapidly and precisely recognize HSO3- being constructed based on the FRET mechanism. DRM-SO2 has extra Large Stokes shift (216 nm), very high sensitivity (DL = 12.2 nM), fast response time and good specificity. When DRM-SO2 undergoes Michael addition with HSO3-, the fluorescence emission peak was blue-shifted from 616 nm to 472 nm, and a clear ratiometric signal appeared. The interaction between lysosomes and mitochondria in maintaining cellular homeostasis was investigated by the dual-targeting ability of the probe using HSO3- as a mediator. DRM-SO2 achieved successful targeting and real-time monitoring of exogenous and endogenous HSO3- in the cells. More importantly, imaging experiments in heat stroke mice revealed high HSO3- expression in intestinal tissues. This provides new ideas and research tools for early prevention of heat stroke-induced diseases such as intestinal injuries. In addition, the semi-quantitative monitoring experiments for paper-based visualization of HSO3- make the probe promising for the design of portable detectors.
基金机构：Science Foundation for Youths Fund of Shanxi Province of China [202203021212388]; Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [2022L256, 2022L398]; Collaborative Innovation Center for Shanxi Advanced Permanent Materials and Technology; The 1331 Engineering of Shanxi Province
基金资助正文：This work was supported by the Science Foundation for Youths Fund of Shanxi Province of China (No. 202203021212388) and Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (No. 2022L256 and No. 2022L398) . This work was also supported by Collaborative Innovation Center for Shanxi Advanced Permanent Materials and Technology and the 1331 Engineering of Shanxi Province.
