Combined serum CTRP7 and CTRP15 levels as a novel biomarker for insulin resistance and type 2 diabetes mellitus
作者全名：Xue, Shiyao; Ling, Jiaxiu; Tian, Mingyuan; Li, Ke; Li, Shengbing; Liu, Dongfang; Li, Ling; Yang, Mengliu; Yang, Gangyi
作者地址：[Xue, Shiyao; Ling, Jiaxiu; Tian, Mingyuan; Li, Ke; Li, Shengbing; Liu, Dongfang; Yang, Mengliu; Yang, Gangyi] Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Chongqing, Peoples R China; [Li, Ling] Chongqing Med Univ, Coll Lab Med, Key Lab Diagnost Med, Minist Educ, Chongqing, Peoples R China; [Li, Ling] Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Chongqing, Peoples R China
通信作者：Yang, GY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Chongqing, Peoples R China.
来源：HELIYON
ESI学科分类：
WOS号：WOS:001239607200001
JCR分区：Q1
影响因子：3.4
年份：2024
卷号：10
期号：9
开始页：
结束页：
文献类型：Article
关键词：CTRP7; CTRP15; Insulin resistance; Interventional tests; T2DM
摘要：Aims: This study aimed to examine the alterations in the serum CTRP7 and CTRP15 concentrations in patients newly diagnosed with type 2 diabetes mellitus (T2DM) and to assess the diagnostic potential of the log10 (CTRP7 +CTRP15) for insulin resistance (IR) and T2DM. Methods: Serum CTRP7, CTRP15, and adiponectin levels were measured using an enzyme -linked immunosorbent assay (ELISA). Bioinformatics analysis was conducted to investigate CTRP7 and CTRP15-related genes and metabolic signaling pathways. Results: Log10 (CTRP7 +CTRP15) levels were notably elevated in the impaired glucose tolerance (IGT) and T2DM cohorts compared with those in the normal control (NGT) cohort. Log10 (CTRP7 +CTRP15) exhibited positive correlations with HOMA-IR, area under the glucose curve (AUCg), HbA1c%, triglyceride (TG), visceral adiposity index (VAI), body mass index (BMI), and free fatty acid (FFA), levels but negative correlations with adiponectin. Multivariate stepwise regression analysis revealed that HOMA-IR, BMI, HbA1c and FFA levels were independent factors affecting the log10 (CTRP7 +CTRP15). Logistic regression analysis revealed that log10 (CTRP7 +CTRP15) was independently associated with T2DM and significantly associated with increased risk. Receiver operating characteristic (ROC) curve analysis indicated that the predictive value of log10 (CTRP7 +CTRP15) for T2DM and IR was superior to that of CTRP7 or CTRP15 alone. Intervention studies demonstrated that insulin, FFAs and acute exercise contribute to the elevation of serum CTRP7 levels, while hyperglycemia inhibited CTRP7 secretion. Short-term changes in blood glucose, insulin, FFA and acute exercise had minimal effects on serum CTRP15 levels. Bioinformatics analysis revealed that CTRP7 and CTRP15 interact with multiple metabolism -related genes and are enriched in glucose and lipid metabolism -related pathways. Conclusion: Log10 (CTRP7 +CTRP15) may serve as a valuable diagnostic marker for the management of metabolic -related diseases, particularly T2DM and IR.
基金机构：Chongqing Postdoctoral Research Funding [2021XM3015]; Kuanren Yingcai Excellent Youth Talents project of the Second Affiliated Hospital of Chongqing Medical University [Kryc-yq-2214]
基金资助正文：The authors express their gratitude to all patients and normal volunteers who participated in this study. This work was supported by research grants from the Chongqing Postdoctoral Research Funding (2021XM3015) and Kuanren Yingcai Excellent Youth Talents project of the Second Affiliated Hospital of Chongqing Medical University (Kryc-yq-2214) .
