Molecular characteristics of Echinococcus multilocularis FABP1 and its regulatory functions on murine macrophages

作者全名：Li, Xiang; Liao, Peng; Zhou, Wenjing; Yang, Xinqi; Ye, Bin

作者地址：[Li, Xiang; Liao, Peng; Zhou, Wenjing; Yang, Xinqi; Ye, Bin] Chongqing Med Univ, Coll Basic Med, Dept Pathogen Biol, Chongqing 400016, Peoples R China

通信作者：Ye, B (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Pathogen Biol, Chongqing 400016, Peoples R China.

来源：ACTA TROPICA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001241209300001

JCR分区：Q2

影响因子：2.1

年份：2024

卷号：255

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Echinococcus multilocularis; Fatty acid binding proteins; Organizational localization; Murine macrophages; Immunomodulatory

摘要：Fatty acid binding proteins (FABPs) have emerged as attractive vaccination candidates for several platyhelminth species. To explore the physiological functions of Echinococcus multilocularis ( E. multilocularis ) FABP, the molecular characteristics of Em FABP1 were analyzed by online software, and the regulatory roles of r Em FABP1 protein in murine macrophages were further investigated. The emfabp1 gene encodes 133 amino acids with the characteristic beta -barrel shape of the cytoplasmic FABP family. Natural Em FABP1 protein is predominantly expressed in protoscoleces tegument and germinal layer cells and is also detected in cyst fluid and exosomes of E. multilocularis . r Em FABP1 protein demonstrated a notable suppression of phagocytic activity and nitric oxide production in murine macrophages. Additionally, the protein was observed to promote apoptosis and regulate cytokine expression in macrophages. These findings suggested that E. multilocularis FABP1 is critical in modifying macrophage physiological processes and that this protein may have immunomodulatory roles during infection.

基金机构：National Natural Science Foundation of China [81672045]

基金资助正文：The authors would like to thank the Research Center for Molecular Medicine and Tumor at Chongqing Medical University for providing laboratory equipments. This work was partially supported by the National Natural Science Foundation of China (grant no. 81672045) .