A xanthan gum and carbomer-codispersed divalent manganese ion-loaded tannic acid nanoparticle adjuvanted inactivated pseudorabies virus vaccine induces balanced humoral and cellular immune responses
作者全名：Guo, Anan; Tang, Lvqing; Yang, Bing; Xie, Niling; Cui, Yandong; Sun, Wen; Li, Yuan; Li, Xiangting; Wu, Yang; Liu, Yingshuai
作者地址：[Guo, Anan; Yang, Bing; Xie, Niling; Cui, Yandong; Li, Xiangting; Liu, Yingshuai] Southwest Univ, Sch Mat & Energy, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China; [Tang, Lvqing; Wu, Yang] China Natl Biotec Grp Adnova Co Ltd, Wuhan 430073, Peoples R China; [Sun, Wen] Sinopharm Anim Hlth Co Ltd, Wuhan 430073, Peoples R China; [Wu, Yang] State Key Lab Novel Vaccines Emerging Infect Dis, Shanghai 100098, Peoples R China; [Li, Yuan] Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing 402160, Peoples R China
通信作者：Liu, YS (通讯作者)，Southwest Univ, Sch Mat & Energy, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China.; Wu, Y (通讯作者)，China Natl Biotec Grp Adnova Co Ltd, Wuhan 430073, Peoples R China.; Sun, W (通讯作者)，Sinopharm Anim Hlth Co Ltd, Wuhan 430073, Peoples R China.; Wu, Y (通讯作者)，State Key Lab Novel Vaccines Emerging Infect Dis, Shanghai 100098, Peoples R China.
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001242298600001
JCR分区：Q1
影响因子：7.7
年份：2024
卷号：269
期号：
开始页：
结束页：
文献类型：Article
关键词：Mn2+; Nanoadjuvant; Immune response; Pseudorabies virus
摘要：Adjuvants including aluminum adjuvant (Alum) and oil -water emulsion have been widely used in inactivated pseudorabies virus (PRV) vaccines to improve their performance, however, they are not sufficient to protect from PRV infection because of the weak immune response and poor Th1-type immune response. Divalent manganese ion (Mn 2 + ) has been reported to increase the cellular immune response significantly. In this work, a xanthan gum and carbomer-dispersed Mn 2 + -loaded tannic acid -polyethylene glycol (TPMnXC) nanoparticle colloid is developed and used as an adjuvant to improve the performance of the inactivated PRV vaccine. The good in vitro and in vivo biocompatibility of the developed TPMnXC colloid has been confirmed by the cell viability assay, erythrocyte hemolysis, blood routine analysis, and histological analysis of mouse organs and injection site. The TPMnXC-adjuvanted inactivated PRV vaccine (TPMnXC@PRV) significantly promotes higher and more balanced immune responses indicating with an increased specific total IgG antibody and IgG2a/IgG1 ratio, efficient splenocytes proliferation, and elevated Th1- and Th2-type cytokine secretion than those of control groups. Wild PRV challenge experiment is performed using mice as a model animal, achieving a protection rate of up to 86.67 %, which is much higher than those observed from the commercial Alum. This work not only demonstrates the high potentiality of TPMnXC in practical applications but also provides a new way to develop the Mn 2 + -loaded nanoadjuvant for veterinary vaccines.
基金机构：The 3551 Optics Valley Innovative Talent Program in 2021 (Wuhan, Hubei); Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease; Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Southwest University, China
基金资助正文：This research was supported by the 3551 Optics Valley Innovative Talent Program in 2021 (Wuhan, Hubei) , Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease and Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Southwest University, China.
