Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma

作者全名：Gu, Dongqing; Zhao, Xin; Song, Jing; Xiao, Jianmei; Zhang, Leida; Deng, Guohong; Li, Dajiang

作者地址：[Gu, Dongqing; Xiao, Jianmei; Deng, Guohong] Army Med Univ, Affiliated Hosp 1, Dept Infect Dis, Chongqing, Peoples R China; [Gu, Dongqing; Xiao, Jianmei; Deng, Guohong] Chongqing Key Lab Viral Infect Dis, Chongqing, Peoples R China; [Zhao, Xin; Zhang, Leida; Li, Dajiang] Army Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Chongqing, Peoples R China; [Song, Jing] Childrens Hosp Chongqing Med Univ CHCMU, Minist Educ, Key Lab Child Dev & Disorders, Chongqing, Peoples R China

通信作者：Deng, GH (通讯作者)，Army Med Univ, Affiliated Hosp 1, Dept Infect Dis, Chongqing, Peoples R China.; Deng, GH (通讯作者)，Chongqing Key Lab Viral Infect Dis, Chongqing, Peoples R China.; Li, DJ (通讯作者)，Army Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Chongqing, Peoples R China.

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001245852100001

JCR分区：Q1

影响因子：5.7

年份：2024

卷号：15

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：cholangiocarcinoma; multiplex immunofluorescence; carcinogenesis; prognosis; IL-6/STAT3 pathway; tissue microarray

摘要：Background Cholangiocarcinoma (CCA) is a typical inflammation-induced malignancy, and elevated serum interleukin-6 (IL-6) levels have been reported to be linked to the onset and progression of CCA. We aim to investigate the potential prognostic value of the IL-6 pathway for CCA.Methods We detected the expressions of IL-6, IL-6R, glycoprotein (gp130), C-reactive protein (CRP), Janus kinase 2 (JAK2), and signal transducer and activator of transcription 3 (STAT3) in CCA tissue microarray using multiplex immunofluorescence. Furthermore, the clinical associations and prognostic values were assessed. Finally, single-cell transcriptome analysis was performed to evaluate the expression level of IL-6 pathway genes in CCA.Results The results revealed that the expression of IL-6 was lower, while the expression of STAT3 was higher in tumor tissues compared to normal tissues. Especially in tumor microenvironment, the expression of IL-6 pathway genes was generally downregulated. Importantly, gp130 was strongly correlated with JAK2 in tumor tissues, while it was moderately correlated with JAK2 in normal tissue. Although none of the gene expressions were directly associated with overall survival and disease-free survival, our study found that IL-6, IL-6R, CRP, gp130, and JAK2 were inversely correlated with vascular invasion, which is a risk factor for poor prognosis in patients with CCA.Conclusion The findings from this study suggest that the IL-6 signaling pathway may have a potential prognostic value for CCA. Further investigation is needed to understand the underlying molecular mechanisms of the IL-6 pathway in CCA.

基金机构：National Natural Science Foundation of China [81903393]

基金资助正文：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (81903393).