Investigation of subclinical ocular inflammation in the aqueous humor of patients with myopia following bilateral sequential collamer lens implantation

作者全名：Wang, Xiao-Qin; Zheng, Shi-Jie; Xiang, Yong-Guo; Huang, Fan -Fan; Huang, Rong-Xi; Wan, Wen-Juan; Hu, Ke

作者地址：[Wang, Xiao-Qin; Zheng, Shi-Jie; Xiang, Yong-Guo; Huang, Fan -Fan; Wan, Wen-Juan; Hu, Ke] Chongqing Med Univ, Chongqing 400016, Peoples R China; [Wang, Xiao-Qin] Peoples Hosp Tongliang Dist, Dept Ophthalmol, Chongqing 400000, Peoples R China; [Zheng, Shi-Jie; Wan, Wen-Juan; Hu, Ke] Chongqing Med Univ, Chongqing Eye Inst, Chongqing Branch,Municipal Div Natl Clin Res Ctr O, Dept ophthalmol,Affiliated Hosp 1,Chongqing Key La, Chongqing 400016, Peoples R China; [Huang, Rong-Xi] Chongqing Peoples Hosp, Dept Endocrinol, Chongqing 400000, Peoples R China

通信作者：Wan, WJ; Hu, K (通讯作者)，Chongqing Med Univ, Chongqing Eye Inst, Chongqing Branch,Municipal Div Natl Clin Res Ctr O, Dept ophthalmol,Affiliated Hosp 1,Chongqing Key La, Chongqing 400016, Peoples R China.

来源：TRANSPLANT IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001245891300001

JCR分区：Q3

影响因子：1.6

年份：2024

卷号：85

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Aqueous humor; Inflammatory factors; Implantable collamer lens (ICL); Subclinical inflammation

摘要：Background: The aqueous humor, a transparent fluid secreted by the ciliary body, supports the lens of the eyeball. In this study, we analyzed the cytokine and chemokine profiles within the aqueous humor of the contralateral eye post-implantation of an implantable collamer lens (ICL) to evaluate potential subclinical inflammation in the second eye subsequent to ICL implantation in the first eye. Methods: Aqueous humor samples were procured from both eyes of 40 patients (totaling 80 eyes) prior to bilateral ICL insertion. Subsequently, a comprehensive statistical analysis was conducted using the Luminex assay to quantify 30 different cytokines in these samples. Results: Compared to the first eye, the aqueous humor of the second eye demonstrated decreased concentrations of IFN-gamma (P = 0.038), IL-13 (P = 0.027), IL-17/IL-17 A (P = 0.012), and IL-4 (P = 0.025). No significant differences were observed in other cytokine levels between the two groups. Patients were then categorized based on the postoperative rise in intraocular pressure (IOP) in the first eye. The group with elevated IOP displayed elevated levels of EGF in the aqueous humor of the first eye (P = 0.013) and higher levels of PDGF-AB/BB in the aqueous humor of the second eye (P = 0.032) compared to the group with normal IOP. Within the elevated IOP group, the levels of EGF (P = 0.013) and IL-17/IL-17 A (P = 0.016) in the aqueous humor were lower in the second eye than in the first eye. In the normal IOP group, cytokine levels did not differ notably between eyes. Conclusion: Following sequential ICL implantation, it appears that a protective response may be activated to mitigate subclinical inflammation in the second eye induced by the initial implantation in the first eye. Additionally, the increase in IOP subsequent to surgery in the first eye may correlate with the presence of inflammatory mediators in the aqueous humor.

基金机构：Science and Technology Commission of China [CSTC2021jscx-gksb-N0017, cstc2021jcyj-msxmX0967]; Chongqing Talent Plan "Contract program" [cstc2022ycjh-bgzxm0121]; Chongqing Science and Health Joint Key Project [2024ZDXM033]; Chongqing Young and Middle-aged High-end Talent Project [2024GDRC005]

基金资助正文：This work was supported by National Natural Science Foundation of China (Grant no. 82371098), the Project Foundation of Chongqing Science and Technology Commission of China (Grant nos. CSTC2021jscx-gksb-N0017 and cstc2021jcyj-msxmX0967), Chongqing Talent Plan "Contract program" (Grant no. cstc2022ycjh-bgzxm0121). Chongqing Science and Health Joint Key Project (Grant no. 2024ZDXM033), Chongqing Young and Middle-aged High-end Talent Project (Grant no. 2024GDRC005).r Science and Technology Commission of China (Grant nos. CSTC2021jscx-gksb-N0017 and cstc2021jcyj-msxmX0967) , Chongqing Talent Plan "Contract program" (Grant no. cstc2022ycjh-bgzxm0121) . Chongqing Science and Health Joint Key Project (Grant no. 2024ZDXM033) , Chongqing Young and Middle-aged High-end Talent Project (Grant no. 2024GDRC005) .