FMOD Alleviates Depression-Like Behaviors by Targeting the PI3K/AKT/mTOR Signaling After Traumatic Brain Injury

作者全名：Huang, Xuekang; Zhu, Ziyu; Du, Mengran; Wu, Chenrui; Fu, Jiayuanyuan; Zhang, Jie; Tan, Weilin; Wu, Biying; Liu, Lian; Liao, Z. B.

作者地址：[Huang, Xuekang; Zhu, Ziyu; Du, Mengran; Wu, Chenrui; Fu, Jiayuanyuan; Zhang, Jie; Tan, Weilin; Wu, Biying; Liu, Lian; Liao, Z. B.] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, 1 Youyi Rd, Chongqing 400016, Peoples R China

通信作者：Liao, ZB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, 1 Youyi Rd, Chongqing 400016, Peoples R China.

来源：NEUROMOLECULAR MEDICINE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001246009700001

JCR分区：Q2

影响因子：3.3

年份：2024

卷号：26

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：TBI; Depression; FMOD; Synaptic plasticity; PI3K/AKT/mTOR

摘要：Depression frequently occurs following traumatic brain injury (TBI). However, the role of Fibromodulin (FMOD) in TBI-related depression is not yet clear. Previous studies have suggested FMOD as a potential key factor in TBI, yet its association with depression post-TBI and underlying mechanisms are not well understood. Serum levels of FMOD were measured in patients with traumatic brain injury using qPCR. The severity of depression was assessed using the self-depression scale (SDS). Neurological function, depressive state, and cognitive function in mice were assessed using the modified Neurological Severity Score (mNSS), forced swimming test (FST), tail suspension test (TST), Sucrose Preference Test (SPT), and morris water maze (MWM). The morphological features of mouse hippocampal synapses and neuronal dendritic spines were revealed through immunofluorescence, transmission electron microscopy, and Golgi-Cox staining. The protein expression levels of FMOD, MAP2, SYP, and PSD95, as well as the phosphorylation levels of the PI3K/AKT/mTOR signaling pathway, were detected through Western blotting. FMOD levels were decreased in TBI patients' serum. Overexpression of FMOD preserved neuronal function and alleviated depression-like behaviour, increased synaptic protein expression, and induced ultrastructural changes in hippocampal neurons. The increased phosphorylation of PI3K, AKT, and mTOR suggested the involvement of the PI3K/AKT/mTOR signaling pathway in FMOD's protective effects. FMOD exhibits potential as a therapeutic target for depression related to TBI, with its protective effects potentially mediated through the PI3K/AKT/mTOR signaling pathway.

基金机构：National Natural Science Foundation of China

基金资助正文：Not applicable.