An Optimized Microwave-Assisted Digestion Method to Analyze the Amino Acids Profile of <i>Quisqualis Fructus</i> from Different Planted Origins

作者全名：Dai, Lei; Yang, Lin; Wang, Yiwu; Li, Yan; Zhao, Jianing; Pan, Shuxiang; Li, Yaxuan; Yang, Dan; He, Dan

作者地址：[Dai, Lei; Zhao, Jianing; Pan, Shuxiang; Li, Yaxuan; Yang, Dan; He, Dan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Yang, Lin; Li, Yan] Chongqing Med & Pharmaceut Coll, Chongqing Pharmaceut Preparat Engn Technol Res Ctr, Chongqing 401331, Peoples R China; [Wang, Yiwu] Chongqing Med Univ, Expt Teaching Ctr, Chongqing 400016, Peoples R China

通信作者：He, D (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China.

来源：FOODS

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:001246816700001

JCR分区：Q1

影响因子：4.7

年份：2024

卷号：13

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：Quisqualis Fructus; microwave-assisted digestion; response surface methodology; amino acids; chemometric; fingerprint

摘要：This study aims to establish a rapid and convenient microwave-assisted digestion method for sample pretreatment to determine amino acid profiles in natural products. This method was applied to analyze the amino acid profiles of Quisqualis Fructus (QF) from different planted origins. The microwave-assisted digestion conditions were optimized by a response surface methodology (RSM), and 17 amino acids in different planted origins of QF were determined by an automatic amino acid analyzer according to the optimized digestion conditions. The contents of 17 amino acids in QF from different planted origins were further analyzed by fingerprint and chemometric analysis. The temperature of microwave digestion at 167 degrees C, time of microwave digestion at 24 min, and a solid-liquid ratio of 46.5 g/mL was selected as the optimal digestion conditions. The total content of 17 amino acids in QF from different planted origins ranged from 71.88 to 91.03 mg/g. Amino acid composition and nutritional evaluation indicated that the content of medicinal amino acids was higher than aromatic amino acids. The results of fingerprint analysis reflected that the similarity between the 16 batches of QF ranged from 0.889 to 0.999, while chemometrics analysis indicated amino acid content in QF varied from different planted origins, and six important differential amino acids were screened. Compared with the traditional extraction method, microwave-assisted digestion with response surface optimized has the advantages of rapidity, convenience, and reliability, which could be used to study the amino acid profiles in natural products. The amino acid profile of QF indicated that it has a rich medicinal nutritional value. Different planted origins of QF have a high degree of similarity and could be effectively distinguished by chemometric analysis.

基金机构：Research Program of Chongqing Graduate Tutor Team Construction Project of Chongqing Municipal Education Commission Foundation

基金资助正文：We thank all partners and laboratory members for their kind help.