Proteomics coupled transcriptomics reveals Slc34a1 and Slc34a3 downregulation as potential features of nephrotoxin-induced acute kidney injury

作者全名：Zhang, Junying; Che, Tiantian; Wang, Liting; Sun, Wei; Zhao, Jing; Chen, Jiajia; Liu, Yang; Pu, Qi; Zhang, Yu; Li, Jiani; Li, Zhangfu; Zhu, Zhaojing; Fu, Qihuan; Wang, Xiaoyang; Yuan, Jiangbei

作者地址：[Zhang, Junying; Zhu, Zhaojing] Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China; [Zhang, Junying] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Che, Tiantian] Chongqing Nanan Dist Ctr Dis Control & Prevent, Chongqing 401336, Peoples R China; [Wang, Liting; Sun, Wei; Zhao, Jing; Chen, Jiajia; Liu, Yang; Pu, Qi; Zhang, Yu; Wang, Xiaoyang] Army Med Univ, Biomed Anal Ctr, Chongqing 400038, Peoples R China; [Li, Jiani; Li, Zhangfu; Yuan, Jiangbei] Peking Univ, Hepatopancreato Biliary Surg, Shenzhen Hosp, Shenzhen 518036, Peoples R China; [Li, Zhangfu; Yuan, Jiangbei] Hong Kong Univ Sci & Technol, Peking Univ, Shenzhen Peking Univ, Hepatopancreato Biliary Surg,Shenzhen Hosp,Med Ct, Shenzhen 518036, Guangdong, Peoples R China; [Fu, Qihuan] Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China; [Yuan, Jiangbei] Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Affiliated Peoples Hosp, Ctr Gen Practice Med,Dept Infect Dis, Hangzhou 310014, Zhejiang, Peoples R China; [Yuan, Jiangbei] Chongqing Med Univ, Dept Infect Dis, Affiliated Banan Hosp, Chongqing 400016, Peoples R China

通信作者：Zhu, ZJ (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China.; Wang, XY (通讯作者)，Army Med Univ, Biomed Anal Ctr, Chongqing 400038, Peoples R China.; Yuan, JB (通讯作者)，Peking Univ, Hepatopancreato Biliary Surg, Shenzhen Hosp, Shenzhen 518036, Peoples R China.; Fu, QH (通讯作者)，Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China.

来源：JOURNAL OF PROTEOMICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001246960700001

JCR分区：Q2

影响因子：2.8

年份：2024

卷号：302

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Quantitative proteomics; RNA sequencing; Acute kidney injury; Slc34a1; Slc34a3; LPS; Cisplatin

摘要：Acute kidney injury (AKI) stands as a prevalent and economically burdensome condition worldwide, yet its complex molecular mechanisms remain incompletely understood. To address this gap, our study employs a multifaceted approach, combining mass spectrometry and RNA sequencing technologies, to elucidate the intricate molecular landscape underlying nephrotoxin-induced AKI in mice by cisplatin- and LPS-induced. By examining the protein and RNA expression profiles, we aimed to uncover novel insights into the pathogenesis of AKI and identify potential diagnostic and therapeutic targets. Our results demonstrate significant downregulation of Slc34a1 and Slc34a3, shedding light on their crucial roles in AKI pathology and highlighting their promise as actionable targets for diagnosis and treatment. This comprehensive analysis not only enhances our understanding of AKI pathophysiology but also offers valuable avenues for the development of targeted interventions to mitigate its clinical impact. Significance: Nephrotoxicity acute kidney injury (AKI) is a common clinical condition whose pathogenesis is the process by which some drugs, chemicals or other factors cause damage to the kidneys, resulting in impaired kidney function. Although it has been proved that different nephrotoxic substances can affect the kidney through different pathways, whether they have a commonality has not been registered. Here, we combined transcriptomics and proteomics to study the molecular mechanism of LPS and cisplatin-induced nephrotoxic acute kidney injury finding that the down -regulation of Slc34a1 and Slc34a3 may be a critical link in nephrotoxic acute kidney injury, which can be used as a marker for its early diagnosis.

基金机构：Shenzhen Science and Technology Innovation Committee [JCYJ20220531094216037, JCYJ20230807095808017]; Science and technology research program of Chongqing Education Commission of China [KJQN202302805]; Natural Science Foundation of Chongqing [2023NSCQ-BHX0377]; School -level project fund of Chongqing Medical and Pharmaceutical College [ygz2022102]

基金资助正文：<STRONG> </STRONG>This work acknowledges the support of the Shenzhen Science and Technology Innovation Committee (No. JCYJ20220531094216037, and No. JCYJ20230807095808017) , the science and technology research program of Chongqing Education Commission of China (No. KJQN202302805) , the Natural Science Foundation of Chongqing (No. 2023NSCQ-BHX0377) , and the school -level project fund of Chongqing Medical and Pharmaceutical College (No. ygz2022102) .