IRTKS contributes to the malignant progression of cervical cancer cells

作者全名：Zhang, Yan; Yi, Faping; Zhang, Xiaoxuan; Song, Jing; Cai, Jing; Lai, Jiayi; Song, Fangzhou

作者地址：[Zhang, Yan; Yi, Faping; Song, Jing; Cai, Jing; Lai, Jiayi; Song, Fangzhou] Chongqing Med Univ, Mol Med & Canc Res Ctr, 1 Med Coll Rd, Chongqing, Peoples R China; [Zhang, Yan; Yi, Faping; Song, Jing; Cai, Jing; Lai, Jiayi; Song, Fangzhou] Chongqing Med Univ, Dept Biochem & Mol Biol, Chongqing, Peoples R China; [Zhang, Xiaoxuan] Chongqing Med Univ, Affiliated Hosp 2, Dept Canc Ctr, Chongqing 400000, Peoples R China

通信作者：Song, FZ (通讯作者)，Chongqing Med Univ, Mol Med & Canc Res Ctr, 1 Med Coll Rd, Chongqing, Peoples R China.; Song, FZ (通讯作者)，Chongqing Med Univ, Dept Biochem & Mol Biol, Chongqing, Peoples R China.

来源：MEDICAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001248219900001

JCR分区：Q2

影响因子：2.8

年份：2024

卷号：41

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：IRTKS; Cervical cancer; Proliferation; Migration; Invasion; EMT

摘要：Cervical cancer (CC), one of the most aggressive tumors in women, has high risk rates of recurrence and metastasis. It is essential to study the key genes and proteins involved in CC development. IRTKS, a member of the IRSp53 family, has been reported as a tumor promoter in gastric and breast cancers. However, the biological role of IRTKS in CC is still unclear. The purpose of this study was to explore the biological function of IRTKS in CC cells in vitro and the effect of IRTKS on tumorigenesis in vivo. Siha and Hela cells were treated with si-RNA and plasmids. Cell proliferation and growth were detected by CCK8, colony formation assay and nude mouse tumorigenicity assay, respectively. Transwell assay was used to analyze cell migration and invasion. The expression of epithelial-mesenchymal transition (EMT)-related proteins was determined by western blot. IRTKS was highly expressed in CC. IRTKS contributed to the proliferation of CC cells in vitro and in vivo. Furthermore, IRTKS facilitated the migration and invasion of CC cells and modulated EMT. IRTKS plays a crucial role in CC tumorigenesis, suggesting it may be a potential key gene for new therapeutic strategies in CC.

基金机构：Chongqing Science and Technology Bureau People's Livelihood Key Project

基金资助正文：No Statement Available