Electroacupuncture enhances cerebral blood perfusion by inhibiting HIF-1α in rat subarachnoid hemorrhage

作者全名：Wang, Yingwen; Chen, Yu; Li, Zhao; Tang, Liuyang; Wen, Daochen; Wu, Yue; Guo, Zongduo

作者地址：[Wang, Yingwen; Wu, Yue; Guo, Zongduo] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, 1 Youyi Rd, Chongqing 400016, Yuzhong, Peoples R China; [Chen, Yu] Chongqing Med Univ, Affiliated Hosp 1, Dept Rehabil, Chongqing 400016, Peoples R China; [Li, Zhao] Chengdu First Peoples Hosp, Dept Emergency, Chengdu 610016, Sichuan, Peoples R China; [Tang, Liuyang] Peoples Hosp, Dept Neurosurg, Chongqing 401420, Qijiang, Peoples R China; [Wen, Daochen] Xuanhan Cty Peoples Hosp, Dept Neurosurg, Dazhou, Peoples R China

通信作者：Wu, Y; Guo, ZD (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, 1 Youyi Rd, Chongqing 400016, Yuzhong, Peoples R China.

来源：BRAIN RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001248473000001

JCR分区：Q3

影响因子：2.7

年份：2024

卷号：1839

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Subarachnoid hemorrhage; Electroacupuncture; Cerebral blood perfusion; HIF-1 alpha; Vasospasm

摘要：Objective: Cerebral blood perfusion (CBP) reduction is a prevalent complication following subarachnoid hemorrhage (SAH) in clinical practice, often associated with long-term cognitive impairment and prognosis. Electroacupuncture (EA), a widely utilized traditional Chinese therapy for central nervous system disorders, has demonstrated promising therapeutic effects. This study aims to investigate the therapeutic potential of EA in restoring CBP in SAH rats and to explore the mechanisms involving HIF-1 alpha in this process. Methods: Rats were randomly assigned to one of five groups, including Sham, SAH, EA, EA + Saline, and EA + dimethyloxallyl glycine (DMOG) groups. EA treatment was administered for 10 min daily, while DMOG were intraperitoneally injected. Behavioral tests, cerebral blood flow monitoring, vascular thickness measurement, western blotting, and immunofluorescence staining were conducted to assess the therapeutic effects of EA on cerebral blood flow. Results: SAH resulted in elevated levels of HIF-1 alpha, endothelin (ET), ICAM-1, P-SELECTIN, E-SELECTIN, and decreased level of eNOS in the brain. This led to cerebral vasospasm, decreased CBF, and cognitive deficits in the rat SAH model. EA intervention downregulated the expression of HIF-1 alpha, ET, ICAM-1, P-SELECTIN, and ESELECTIN, while increasing eNOS expression. This alleviated cerebral vasospasm, restored CBF, and improved cognitive function. However, the administration of the HIF-1 alpha stabilizer (DMOG) counteracted the therapeutic effects of EA. Conclusion: EA promotes the recovery of cerebral blood flow after SAH injury, attenuates cerebral vasospasm, and accelerates the recovery of cognitive dysfunction, and its mechanism of action may be related to the inhibition of the HIF-1 alpha signaling pathway.

基金机构：National Natural Science Foundation of China [82071332, 81901210]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN202000428, CYS22342]

基金资助正文：This work was supported by the National Natural Science Foundation of China (No.82071332 and No.81901210), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No.KJQN202000428 and Grant No.CYS22342).