Landscape of infiltrated immune cell characterization in COVID-19

作者全名：Zheng, Wei; Zhang, Yuan; Lai, Guichuan; Xie, Biao

作者地址：[Zheng, Wei] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Zhang, Yuan; Lai, Guichuan; Xie, Biao] Chongqing Med Univ, Sch Publ Hlth, Dept Epidemiol & Hlth Stat, Yixue Rd, Chongqing 400016, Peoples R China

通信作者：Lai, GC; Xie, B (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Epidemiol & Hlth Stat, Yixue Rd, Chongqing 400016, Peoples R China.

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001251079300001

JCR分区：Q1

影响因子：3.4

年份：2024

卷号：10

期号：6

开始页：　

结束页：　

文献类型：Article

关键词：Coronavirus disease 2019; Immune infiltrating cells; Immune response; Gene expression; Viral infection; Severity

摘要：Purpose: Although the role of SARS-CoV-2-specfic immune cells has been revealed, a comprehensive understanding of immune patterns remains unknown. Methods: In this work, unsupervised consensus clustering analysis was used to classify 240 coronavirus disease 2019 (COVID-19) patients into different immune subtypes. Next, we performed differentially expressed analysis between different immune subtypes. Functional enrichment and pathway analyses were employed to reveal the biological significance of these differentially expressed genes (DEGs). Besides, we compared feature score of some DEGs between whole blood and lung tissues. Then, we utilized the "GSVA" algorithm to construct an immune cell infiltrating (ICI) tool based on the categories of these DEGs. Finally, we developed a nomogram associated with severity of COVID-19. Results: As a result, we identified two immune subtypes, and 238 DEGs which mainly participated in some immune-related functions and the COVID-19 pathway. Most importantly, the 238 DEGs could reflect the characterization of immune patterns in lung tissues. ICI scores were markedly negative associated with immune scores. It was worth noting that ICI score was a strong indicator for severity of COVID-19 and could accurately predict the severity of COVID-19. Conclusion: Our findings could provide more valuable strategies for the management of COVID19.

基金机构：National Youth Science Foundation Project, China [82204159]; Science and Technology Research Program of Chongqing Municipal Education Commission, China [KJQN202300423]

基金资助正文：<BOLD>Ethics declarations </BOLD> This research was funded by the National Youth Science Foundation Project, China grant number (82204159) , and Science and Technology Research Program of Chongqing Municipal Education Commission, China (Grant No. KJQN202300423) .