Dihydroartemisinin inhibits HNSCC invasion and migration by controlling miR-195-5p expression

作者全名：Wu, Xiaolu; Yang, Congwen; Li, Zhongwan; Lv, Ping; An, Xiang; Peng, Xiaohe; Li, You; Jiang, Xiaojun; Mao, Xuemei; Chen, Donghong; Jia, Lifeng; Yuan, Wei

作者地址：[Wu, Xiaolu; Yuan, Wei] North Sichuan Med Coll Univ, Nanchong, Peoples R China; [Wu, Xiaolu; An, Xiang; Chen, Donghong; Jia, Lifeng; Yuan, Wei] Chongqing Univ, Chongqing Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Chongqing, Peoples R China; [Peng, Xiaohe; Li, You; Jiang, Xiaojun] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing, Peoples R China; [Lv, Ping] North Sichuan Med Coll, Affiliated Hosp, Nanchong, Peoples R China; [Jiang, Xiaojun] Chongqing Fifth Peoples Hosp, Chongqing, Peoples R China; [Mao, Xuemei] Chongqing Med Univ, Univ Town Hosp, Chongqing, Peoples R China

通信作者：Jia, LF; Yuan, W (通讯作者)，Chongqing Univ, Chongqing Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Chongqing, Peoples R China.

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001251300200001

JCR分区：Q1

影响因子：3.4

年份：2024

卷号：10

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：Dihydroartemisinin (DHA); Head and neck squamous cell carcinoma; miR-195-5p; Teneurin transmembrane protein 2; Invasion; Migration

摘要：Objectives: Dihydroartemisinin (DHA), an artemisinin derivative extracted from the traditional Chinese medicinal herb Artemisia annua , has the potential to suppress head and neck squamous cell carcinoma (HNSCC) progression. However, the mechanisms underlying these effects remain unclear. Therefore, we aimed to examine the mechanisms underlying the effects of DHA on tumor invasion and migration. Methods: Human HNSCC cell lines CAL -27 and FaDu were exposed to varying DHA concentrations (0, 5, 20, and 80 mu M) for 24 h. Cell proliferation, invasion, and migration were assessed using CCK8, transwell, and wound -healing assays, respectively. Quantitative real-time PCR, western blotting, and immunofluorescence were used to assess the expression levels of the target genes and proteins. Results: DHA suppressed the invasion and migration of CAL -27 and FaDu cells. Additionally, miR195-5p suppressed the invasion and migration of HNSCC cells. This study revealed significant differences in the expression of miR-195-5p and TENM2 between clinical samples and multiple public databases. DHA treatment and miR-195-5p overexpression significantly reduced TENM2 expression in HNSCC cells, which suggested that miR-195-5p overexpression enhanced the inhibitory effect of DHA on TENM2. Conclusions: This study provides the first evidence that DHA inhibits cell invasion and migration by regulating the miR-195-5p/TENM2 axis in HNSCC cells, suggesting it as a potentially effective treatment strategy for HNSCC.

基金机构：National Natural Science Foundation of China [81873702]; Natural Science Foundation of Chongqing Municipality [CSTB2022NSCQ-MSX0553, cstc2020jcyj-msxmX0509]; Science -Health Joint Medical Scientific Research Project of Chongqing [2021MSXM018, 2022DBXM006, cstc2022ycjh-bgzxm0126, CSTB2023TIAD- KPX0059, 2000109-02]

基金资助正文：This work was supported by National Natural Science Foundation of China (NO. 81873702) , Natural Science Foundation of Chongqing Municipality (NO. CSTB2022NSCQ-MSX0553, NO. cstc2020jcyj-msxmX0509) and Science -Health Joint Medical Scientific Research Project of Chongqing (NO. 2021MSXM018, NO. 2022DBXM006, NO. cstc2022ycjh-bgzxm0126, NO. CSTB2023TIAD- KPX0059, NO. 2000109-02) .