Downregulation of DIP2B as a prognostic marker inhibited cancer proliferation and migration and was associated with immune infiltration in lung adenocarcinoma via CCND1 and MMP2
作者全名：Wu, Chuang-Yan; Liu, Zhao; Luo, Wei-Min; Huang, Huan; Jiang, Ni; Du, Zhi-Peng; Wang, Fang-Ming; Han, Xu; Ye, Guan-Chao; Guo, Qiang; Chen, Jiu-Ling
作者地址：[Wu, Chuang-Yan; Wang, Fang-Ming; Ye, Guan-Chao; Chen, Jiu-Ling] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Thorac Surg, Wuhan, Peoples R China; [Liu, Zhao] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Canc Ctr, Wuhan, Peoples R China; [Luo, Wei-Min; Guo, Qiang] Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Peoples R China; [Huang, Huan] Peoples Hosp Dongxihu, Dept Thorac Surg, Wuhan, Peoples R China; [Jiang, Ni] Chongqing Med Univ, Women & Childrens Hosp, Dept Obstet & Gynecol, Chongqing, Peoples R China; [Du, Zhi-Peng; Han, Xu] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Gastroenterol, Wuhan, Peoples R China
通信作者：Chen, JL (通讯作者)，Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Thorac Surg, Wuhan, Peoples R China.; Guo, Q (通讯作者)，Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Peoples R China.
来源：HELIYON
ESI学科分类：
WOS号：WOS:001251997100001
JCR分区：Q1
影响因子：3.4
年份：2024
卷号：10
期号：12
开始页：
结束页：
文献类型：Article
关键词：DIP2B; Biomarker; Lung adenocarcinoma; Immune microenvironment; Prognosis
摘要：Background: DIP2B is related to cancer progression. This study investigated the roles and pathways of DIP2B in lung adenocarcinoma (LUAD). Methods: DIP2B expression and the relationship between survival time of cancer patients and DIP2B expression were analyzed. The relationship between DIP2B expression and survival time in LUAD patients was evaluated by a meta-analysis. Cox and survival analyses were used to evaluate the prognostic factors and construct a prognostic nomogram. The mechanisms and effects of DIP2B and the relationship between DIP2B expression and the immune microenvironment were investigated using bioinformatics, CCK-8, western blotting, and transwell experiments. Results: DIP2B was overexpressed in LUAD tissues. DIP2B overexpression was associated with shorter prognosis and was an unfavorable risk factor for prognosis in LUAD patients. DIP2B coexpressed genes were involved in cell division, DNA repair, cell cycle, and others. Inhibition of DIP2B expression could downregulate the proliferation, migration, and invasion of LUAD A549 and H1299 cells, which was related to the decrease in CCND1 and MMP2 protein expression. BRCA1 overexpression was associated with short prognosis, and the nomogram formed by DIP2B and BRCA1 was associated with a poor prognosis in LUAD patients. DIP2B expression correlated with immune cells (such as CD8 T cells, Tcm, and iDCs) and cell markers. Conclusion: DIP2B is a potential biomarker of poor prognosis and the immune microenvironment in LUAD. Inhibition of DIP2B expression downregulated cancer cell proliferation, migration, and invasion, which might be related to the decrease in CCND1 and MMP2 protein expression. DIP2Brelated nomograms might be useful tools for predicting the prognosis of LUAD patients.
基金机构：Natural Science Foundation of China [82100115, 82100299]; Health Commission of Hubei Provincial [WJ2023M166]; Natural Science Foundation of Hubei [2024AFB694]
基金资助正文：This research was funded via the Natural Science Foundation of China (No. 82100115 and 82100299) , the Health Commission of Hubei Provincial (No. WJ2023M166) , and the Natural Science Foundation of Hubei (No. 2024AFB694) .
