Small non-coding RNA signatures in atrial appendages of patients with atrial fibrillation

作者全名：Zeng, Yuhong; Yuan, Zhiquan; Li, Jun; Yang, Lanqing; Li, Chengying; Xiang, Ying; Wu, Long; Xia, Tingting; Zhong, Li; Li, Yafei; Wu, Na

作者地址：[Zeng, Yuhong; Yuan, Zhiquan; Yang, Lanqing; Li, Chengying; Xiang, Ying; Wu, Long; Xia, Tingting; Li, Yafei; Wu, Na] Third Mil Med Univ, Army Med Univ, Coll Prevent Med, Dept Epidemiol, Chongqing 400038, Peoples R China; [Li, Jun] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Thorac & Cardiac Surg,Affiliated Hosp 1, Chongqing, Peoples R China; [Zhong, Li] Chongqing Med Univ, Affiliated Hosp 3, Cardiovasc Dis Ctr, Chongqing 401120, Peoples R China

通信作者：Li, YF; Wu, N (通讯作者)，Third Mil Med Univ, Army Med Univ, Coll Prevent Med, Dept Epidemiol, Chongqing 400038, Peoples R China.; Zhong, L (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Cardiovasc Dis Ctr, Chongqing 401120, Peoples R China.

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001252381000001

JCR分区：Q2

影响因子：4.3

年份：2024

卷号：28

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：atrial fibrillation; ribosomal RNA-derived small RNAs; small non-coding RNAs; small nucleolar RNAs; transfer RNA-derived small RNAs

摘要：The development of high-throughput technologies has enhanced our understanding of small non-coding RNAs (sncRNAs) and their crucial roles in various diseases, including atrial fibrillation (AF). This study aimed to systematically delineate sncRNA profiles in AF patients. PANDORA-sequencing was used to examine the sncRNA profiles of atrial appendage tissues from AF and non-AF patients. Differentially expressed sncRNAs were identified using the R package DEGseq 2 with a fold change >2 and p < 0.05. The target genes of the differentially expressed sncRNAs were predicted using MiRanda and RNAhybrid. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. In AF patients, the most abundant sncRNAs were ribosomal RNA-derived small RNAs (rsRNAs), followed by transfer RNA-derived small RNAs (tsRNAs), and microRNAs (miRNAs). Compared with non-AF patients, 656 rsRNAs, 45 miRNAs, 191 tsRNAs and 51 small nucleolar RNAs (snoRNAs) were differentially expressed in AF patients, whereas no significantly differentially expressed piwi-interacting RNAs were identified. Two out of three tsRNAs were confirmed to be upregulated in AF patients by quantitative reverse transcriptase polymerase chain reaction, and higher plasma levels of tsRNA 5006c-LysCTT were associated with a 2.55-fold increased risk of all-cause death in AF patients (hazard ratio: 2.55; 95% confidence interval, 1.56-4.17; p < 0.001). Combined with our previous transcriptome sequencing results, 32 miRNA, 31 snoRNA, 110 nucleus-encoded tsRNA, and 33 mitochondria-encoded tsRNA target genes were dysregulated in AF patients. GO and KEGG analyses revealed enrichment of differentially expressed sncRNA target genes in AF-related pathways, including the 'calcium signaling pathway' and 'adrenergic signaling in cardiomyocytes.' The dysregulated sncRNA profiles in AF patients suggest their potential regulatory roles in AF pathogenesis. Further research is needed to investigate the specific mechanisms of sncRNAs in the development of AF and to explore potential biomarkers for AF treatment and prognosis.

基金机构：National Natural Science Foundation of China

基金资助正文：None declared.