A syringe-driven PicoArray device for rapid isolation and quantification of foodborne Salmonella

作者全名：Yang, Sa; Hu, Tianbao; Zhang, Qi; Sun, Bangyong; Qin, Zhiyou; Zheng, Kaiyuan; Liu, Yanwu; Li, Gang

作者地址：[Yang, Sa; Hu, Tianbao; Zhang, Qi; Qin, Zhiyou; Zheng, Kaiyuan; Li, Gang] Chongqing Univ, Key Lab Optoelect Technol & Syst, Def Key Disciplines Lab Novel Micronano Devices &, Minist Educ, Chongqing 400044, Peoples R China; [Sun, Bangyong] Xinjiang Univ, Sch Future Technol, Urumqi 830017, Peoples R China; [Liu, Yanwu] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China

通信作者：Li, G (通讯作者)，Chongqing Univ, Key Lab Optoelect Technol & Syst, Def Key Disciplines Lab Novel Micronano Devices &, Minist Educ, Chongqing 400044, Peoples R China.; Liu, YW (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China.

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:001253089700001

JCR分区：Q1

影响因子：8

年份：2024

卷号：417

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Syringe-vacuum; PicoArray device; Isolation; Bacteria enumeration; Salmonella

摘要：Rapid and accurate detection of pathogenic Salmonella in food and environmental sources is of paramount importance to protect public health from salmonellosis. However, most current assays are labor-intensive, timeconsuming, and require expensive instruments and experienced operators. In this work, we developed a PicoArray-based colony-forming unit (CFU) quantification system for rapid and ultrasensitive Salmonella detection. To enable on-site detection, we employed syringe-vacuum-assisted sample loading and oil-induced sample digitization to stochastically confine single bacteria in the picoliter-level wells of the PicoArray device (containing 40,233 wells). Such a single bacterial confinement enables the isolation and characterization of individual bacteria from a large population. In addition, the confinement of bacteria in small volumes allows rapid accumulation of metabolites, thereby eliminating the pre-incubation step and reducing the time required to detect bacteria. By combining it with a selective medium for the isolation of Salmonella and a fluorescent redox indicator (e.g., resazurin) to facilitate the detection of individual microcolonies, the PicoArray device can rapidly and precisely detect Salmonella. As a proof-of-concept, we demonstrated the ability of our platform for rapid, sensitive, and specific detection of Salmonella in spiked milk samples. Our platform offers the possibility of close monitoring of pathogens in food or drinks at the consumer end because it allows a non-trained operator to detect foodborne pathogens in the clinically relevant range in the home setting.

基金机构：National Natural Science Foundation of China [61974012, 21827812]; Natural Sci- ence Foundation of Chongqing [cstc2020jcyj-msxmX0188]; Natural Science Foundation of Xinjiang Uygur Autonomous Region [2023D01C179]; Xinjiang Tianchi Yingcai Youth Doctoral Project [51052300578]

基金资助正文：This work was supported by grants from the National Natural Science Foundation of China (No. 61974012 and 21827812) , the Natural Sci- ence Foundation of Chongqing (No. cstc2020jcyj-msxmX0188) , the Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 2023D01C179) , and Xinjiang Tianchi Yingcai Youth Doctoral Project (No. 51052300578) .