Serum Metabolomics Reveals Metabolomic Profile and Potential Biomarkers in Asthma
作者全名：Zhu, Tao; Ma, Yuan; Wang, Jiajia; Xiong, Wei; Mao, Ruolin; Cui, Bo; Min, Zhihui; Song, Yuanlin; Chen, Zhihong
作者地址：[Zhu, Tao] Suining Cent Hosp, Dept Resp Med & Crit Care Med, Suining, Peoples R China; [Zhu, Tao] Suining Cent Hosp, Preclin Res Ctr, Suining, Peoples R China; [Ma, Yuan; Mao, Ruolin; Cui, Bo; Song, Yuanlin; Chen, Zhihong] Fudan Univ, Zhongshan Hosp, Shanghai Inst Resp Dis, Dept Resp & Crit Care Med, 180 Fenglin Rd, Shanghai 200032, Peoples R China; [Wang, Jiajia] Chongqing Med Univ, Affiliated Hosp 2, Rheumatol Med, Chongqing, Peoples R China; [Xiong, Wei] Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med & Crit Care Med, Chongqing, Peoples R China; [Min, Zhihui] Fudan Univ, Zhongshan Hosp, Res Ctr, Shanghai, Peoples R China
通信作者：Song, YL; Chen, ZH (通讯作者)，Fudan Univ, Zhongshan Hosp, Shanghai Inst Resp Dis, Dept Resp & Crit Care Med, 180 Fenglin Rd, Shanghai 200032, Peoples R China.
来源：ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
ESI学科分类：IMMUNOLOGY
WOS号：WOS:001253172500004
JCR分区：Q2
影响因子：4.1
年份：2024
卷号：16
期号：3
开始页：235
结束页：252
文献类型：Article
关键词：Asthma; metabolomics; metabolites; pathogenesis; diagnosis
摘要：Purpose: Asthma is a highly heterogeneous disease. Metabolomics plays a pivotal role in the pathogenesis and development of asthma. The main aims of our study were to explore the underlying mechanism of asthma and to identify novel biomarkers through metabolomics approach. Methods: Serum samples from 102 asthmatic patients and 18 healthy controls were collected and analyzed using liquid chromatography -mass spectrometry/mass spectrometry (LCMS/MS) system. Multivariate analysis and weighted gene co -expression network analysis (WGCNA) were performed to explore asthma -associated metabolomics profile and metabolites. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for pathway enrichment analysis. Subsequently, 2 selected serum hub metabolites, myristoleic acid and dodecanoylcarnitine, were replicated in a validation cohort using ultra -high performance LCMS/MS system (UHPLC-MS/MS). Results: Distinct metabolomics profile of asthma was revealed by multivariate analysis. Then, 116 overlapped asthma -associated metabolites between multivariate analysis and WGCNA, including 12 hub metabolites, were identified. Clinical features -associated hub metabolites were also identified by WGCNA. Among 116 asthma -associated metabolites, Sphingolipid metabolism and valine, leucine and isoleucine biosynthesis were revealed by KEGG analysis. Furthermore, serum myristoleic acid and dodecanoylcarnitine were significantly higher in asthmatic patients than in healthy controls in validation cohort. Additionally, serum myristoleic acid and dodecanoylcarnitine demonstrated high sensitivities and specificities in predicting asthma. Conclusions: Collectively, asthmatic patients showed a unique serum metabolome. Sphingolipid metabolism and valine, leucine and isoleucine biosynthesis were involved in the pathogenesis of asthma. Furthermore, our results suggest the promising values of serum myristoleic acid and dodecanoylcarnitine for asthma diagnosis in adults.
基金机构：Health Commission Technology Project of Sichuan [23NSFSC0667]; Respiratory Diseases Youth Practical Research Project of China International Medical Exchange Foundation [23LCYJ008]; [Z-2017-24-2301]
基金资助正文：This study was supported by National Natural Science Foundation of China (82270026 and 81970023) , Shanghai Top-Priority Clinical Key Disciplines Construction Project (2017ZZ02013) , Shanghai Municipal Key Clinical Specialty (shslczdzk02201) , Chongqing Health Joint Medical Research Project (2020MSXM112) , Natural Sciences Foundation of Sichuan (23NSFSC0667) , Health Commission Technology Project of Sichuan (23LCYJ008) , and Respiratory Diseases Youth Practical Research Project of China International Medical Exchange Foundation (Z-2017-24-2301) .r Sichuan (23NSFSC0667) , Health Commission Technology Project of Sichuan (23LCYJ008) , and Respiratory Diseases Youth Practical Research Project of China International Medical Exchange Foundation (Z-2017-24-2301) .
