A novel "on-off-on" electrochemiluminescence aptasensor based on resonance energy transfer as signal amplification strategy for ultrasensitive detection of sulfadiazine in different samples

作者全名：Long, Youqi; Liu, Junjie; Wu, Yijie; Fu, Lin; Bai, Lijuan

作者地址：[Long, Youqi] Chongqing Med Univ, Univ Town Hosp, Dept Pharm, Chongqing 401331, Peoples R China; [Liu, Junjie; Wu, Yijie; Fu, Lin; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China

通信作者：Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China.

来源：FOOD CHEMISTRY

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:001254386600001

JCR分区：Q1

影响因子：8.5

年份：2024

卷号：456

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Antibiotics; Emitter; Metal-organic framework; Quenching probe

摘要：The misuse of antibiotics may contaminate the environment and cause harm to human health. Therefore, rapid and accurate detection of antibiotics is essential. In this study, a novel electrochemiluminescence resonance energy transfer (ECL-RET) pair was designed using a new ECL emitter (CPM, Ce-TBAPy) as the donor and CoMOF@AuPt as the acceptor. Moreover, a highly sensitive and specific "on-off-on" ECL aptasensor was constructed for detecting sulfadiazine (SDZ). The aptasensor exhibited a broad linear range (from 10.0 fg mL-1 to 100 ng mL-1) for the SDZ concentration, with limit of detection and limit of quantification values of 1.14 fg mL- 1and 3.75 fg mL-1, respectively. The aptasensor achieved good results in spiking experiments with milk and egg samples, and successfully quantified SDZ in fish meal quality control sample. The prepared aptasensor presents great potential for food and environmental safety by detecting antibiotics.

基金机构：National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Ba Yu Scholar Program, China [YS2019020]

基金资助正文：This work is supported by National Natural Science Foundation of China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) and Discipline Talent Training Pro- gram of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) .