iPSCs chondrogenic differentiation for personalized regenerative medicine: a literature review
作者全名：Ali, Eltahir Abdelrazig Mohamed; Smaida, Rana; Meyer, Morgane; Ou, Wenxin; Li, Zongjin; Han, Zhongchao; Benkirane-Jessel, Nadia; Gottenberg, Jacques Eric; Hua, Guoqiang
作者地址：[Ali, Eltahir Abdelrazig Mohamed; Benkirane-Jessel, Nadia; Hua, Guoqiang] Inst Natl St & Rech Medicale INSERM, UMR 1260, Regenerat Nanomed RNM, 1 Rue Eugene Boeckel, F-67000 Strasbourg, France; [Ali, Eltahir Abdelrazig Mohamed; Meyer, Morgane; Ou, Wenxin; Benkirane-Jessel, Nadia; Gottenberg, Jacques Eric; Hua, Guoqiang] Univ Strasbourg, F-67000 Strasbourg, France; [Smaida, Rana; Meyer, Morgane; Benkirane-Jessel, Nadia] Lamina Therapeut, 1 Rue Eugene Boeckel, F-67000 Strasbourg, France; [Li, Zongjin] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China; [Han, Zhongchao] Beijing Inst Hlth & Stem Cells Hlth & Biotech Co, Beijing Engn Lab Perinatal Stem Cells, Beijing 100176, Peoples R China; [Ou, Wenxin; Gottenberg, Jacques Eric] Ctr Hosp Univ Strasbourg, Ctr Natl Reference Malad Autoimmunes & Syst Rares, Serv Rhumatol, Est Sud Ouest RESO, F-67000 Strasbourg, France; [Ou, Wenxin] Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China
通信作者：Benkirane-Jessel, N; Hua, GQ (通讯作者)，Inst Natl St & Rech Medicale INSERM, UMR 1260, Regenerat Nanomed RNM, 1 Rue Eugene Boeckel, F-67000 Strasbourg, France.; Benkirane-Jessel, N; Gottenberg, JE; Hua, GQ (通讯作者)，Univ Strasbourg, F-67000 Strasbourg, France.; Benkirane-Jessel, N (通讯作者)，Lamina Therapeut, 1 Rue Eugene Boeckel, F-67000 Strasbourg, France.; Gottenberg, JE (通讯作者)，Ctr Hosp Univ Strasbourg, Ctr Natl Reference Malad Autoimmunes & Syst Rares, Serv Rhumatol, Est Sud Ouest RESO, F-67000 Strasbourg, France.
来源：STEM CELL RESEARCH & THERAPY
ESI学科分类：MOLECULAR BIOLOGY & GENETICS
WOS号：WOS:001255397900001
JCR分区：Q1
影响因子：7.1
年份：2024
卷号：15
期号：1
开始页：
结束页：
文献类型：Review
关键词：Induced pluripotent stem cells; Chondrocytes; Mesenchymal stem cells; Osteoarthritis; Cartilage regeneration; Personalized regenerative medicine
摘要：Cartilage, an important connective tissue, provides structural support to other body tissues, and serves as a cushion against impacts throughout the body. Found at the end of the bones, cartilage decreases friction and averts bone-on-bone contact during joint movement. Therefore, defects of cartilage can result from natural wear and tear, or from traumatic events, such as injuries or sudden changes in direction during sports activities. Overtime, these cartilage defects which do not always produce immediate symptoms, could lead to severe clinical pathologies. The emergence of induced pluripotent stem cells (iPSCs) has revolutionized the field of regenerative medicine, providing a promising platform for generating various cell types for therapeutic applications. Thus, chondrocytes differentiated from iPSCs become a promising avenue for non-invasive clinical interventions for cartilage injuries and diseases. In this review, we aim to highlight the current strategies used for in vitro chondrogenic differentiation of iPSCs and to explore their multifaceted applications in disease modeling, drug screening, and personalized regenerative medicine. Achieving abundant functional iPSC-derived chondrocytes requires optimization of culture conditions, incorporating specific growth factors, and precise temporal control. Continual improvements in differentiation methods and integration of emerging genome editing, organoids, and 3D bioprinting technologies will enhance the translational applications of iPSC-derived chondrocytes. Finally, to unlock the benefits for patients suffering from cartilage diseases through iPSCs-derived technologies in chondrogenesis, automatic cell therapy manufacturing systems will not only reduce human intervention and ensure sterile processes within isolator-like platforms to minimize contamination risks, but also provide customized production processes with enhanced scalability and efficiency.
基金机构：Institut national de la sante et de la recherche medicale (INSERM); Faculte de medecine et Faculte de chirurgie dentaire de Universite de Strasbourg; Lamina therapeutics; ANR ARTITHERA; Chinese Scholarship Council (CSC) [202309240005]
基金资助正文：We thank the support of Institut national de la sante et de la recherche medicale (INSERM), Faculte de medecine et Faculte de chirurgie dentaire de Universite de Strasbourg, and Lamina therapeutics. EAMA is financially supported by ANR ARTITHERA, WO was supported by Chinese Scholarship Council (CSC N degrees 202309240005). We also thank Servier Medical ART for free medical images.
