The Nucleolar Protein C1orf131 Is a Novel Gene Involved in the Progression of Lung Adenocarcinoma Cells through the AKT Signalling Pathway

作者全名：Wei, Zhili; Zhao, Yiming; Cai, Jing; Xie, Yajun

作者地址：[Wei, Zhili; Xie, Yajun] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China; [Zhao, Yiming] Chongqing Med Univ, Coll Med Informat, Chongqing 400016, Peoples R China; [Cai, Jing] Harbin Med Univ, Coll Basic Med, Natl Talent Intro Demonstrat Base, Harbin 150081, Peoples R China

通信作者：Xie, YJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China.

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:001256541700001

JCR分区：Q1

影响因子：4.9

年份：2024

卷号：25

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：C1orf131; nucleolar protein; lung cancer; cell cycle; proliferation

摘要：Lung adenocarcinoma (LUAD) is the most widespread cancer in the world, and its development is associated with complex biological mechanisms that are poorly understood. Here, we revealed a marked upregulation in the mRNA level of C1orf131 in LUAD samples compared to non-tumor tissue samples in The Cancer Genome Atlas (TCGA). Depletion of C1orf131 suppressed cell proliferation and growth, whereas it stimulated apoptosis in LUAD cells. Mechanistic investigations revealed that C1orf131 knockdown induced cell cycle dysregulation via the AKT and p53/p21 signalling pathways. Additionally, C1orf131 knockdown blocked cell migration through the modulation of epithelial-mesenchymal transition (EMT) in lung adenocarcinoma. Notably, we identified the C1orf131 protein nucleolar localization sequence, which included amino acid residues 137-142 (KKRKLT) and 240-245 (KKKRKG). Collectively, C1orf131 has potential as a novel therapeutic marker for patients in the future, as it plays a vital role in the progression of lung adenocarcinoma.

基金机构：National Natural Science Funds of China [32270834]; Basic Science and Frontier Technology Research Program of Chongqing Science and Technology Commission [cstc2021jcyj-msxmX0312]; Top-notch Talents Program for Graduate Students of Chongqing Medical University [BJRC202211]

基金资助正文：This research was funded by the National Natural Science Funds of China (grant number:32270834 ), the Basic Science and Frontier Technology Research Program of Chongqing Science and Technology Commission (grant number: cstc2021jcyj-msxmX0312) and the Top-notch Talents Program for Graduate Students of Chongqing Medical University (grant number: BJRC202211).