Comprehensive analysis of key host gene-microbe networks in the cecum tissues of the obese rabbits induced by a high-fat diet

作者全名：Li, Yanhong; Qi, Xiaolan; Wang, Qinrong; He, Yan; Li, Zhupeng; Cen, Xi; Wei, Limin

作者地址：[Li, Yanhong; Qi, Xiaolan; Wang, Qinrong; He, Yan; Li, Zhupeng; Cen, Xi] Guizhou Med Univ, Key Lab Endem & Ethn Dis, Minist Educ, Guiyang, Guizhou, Peoples R China; [Li, Yanhong; Qi, Xiaolan; Wang, Qinrong; He, Yan; Li, Zhupeng; Cen, Xi] Guizhou Med Univ, Collaborat Innovat Ctr Prevent & Control Endem & E, Key Lab Med Mol Biol Guizhou Prov, Guiyang, Guizhou, Peoples R China; [Wei, Limin] Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing, Peoples R China; [Wei, Limin] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China

通信作者：Wei, LM (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing, Peoples R China.; Wei, LM (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China.

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001257037500001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：lipid metabolism; cecum microorganism; fattening model of rabbit; obesity; metagenome

摘要：The Cecum is a key site for cellulose digestion in nutrient metabolism of intestine, but its mechanisms of microbial and gene interactions has not been fully elucidated during pathogenesis of obesity. Therefore, the cecum tissues of the New Zealand rabbits and their contents between the high-fat diet-induced group (Ob) and control group (Co) were collected and analyzed using multi-omics. The metagenomic analysis indicated that the relative abundances of Corallococcus_sp._CAG:1435 and Flavobacteriales bacterium species were significantly lower, while those of Akkermansia glycaniphila, Clostridium_sp._CAG:793, Mycoplasma_sp._CAG:776, Mycoplasma_sp._CAG:472, Clostridium_sp._CAG:609, Akkermansia_sp._KLE1605, Clostridium_sp._CAG:508, and Firmicutes_bacterium_CAG:460 species were significantly higher in the Ob as compared to those in Co. Transcriptomic sequencing results showed that the differentially upregulated genes were mainly enriched in pathways, including calcium signaling pathway, PI3K-Akt signaling pathway, and Wnt signaling pathway, while the differentially downregulated genes were mainly enriched in pathways of NF-kappaB signaling pathway and T cell receptor signaling pathway. The comparative analysis of metabolites showed that the glycine, serine, and threonine metabolism and cysteine and methionine metabolism were the important metabolic pathways between the two groups. The combined analysis showed that CAMK1, IGFBP6, and IGFBP4 genes were highly correlated with Clostridium_sp._CAG:793, and Akkermansia_glycaniphila species. Thus, the preliminary study elucidated the microbial and gene interactions in cecum of obese rabbit and provided a basis for further studies in intestinal intervention for human obesity.

基金机构：Guizhou Medical University [RN2022-GK017, TJ23059]; Natural Science Foundation of China [32360154]

基金资助正文：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by research initiating fund of Guizhou Medical University,grant number RN2022-GK017 the research project (TJ23059) and Natural Science Foundation of China (32360154)