Screening of potential hub genes involved in Kidney Wilms tumor via bioinformatics analysis and experimental validation

作者全名：Zeng, Qiang; Liu, Tingting; Qin, Lilu; Wang, Chen; Peng, Guangbei; Liu, Zhong; Tao, Junfeng

作者地址：[Zeng, Qiang; Liu, Tingting; Qin, Lilu; Wang, Chen; Peng, Guangbei; Liu, Zhong; Tao, Junfeng] Jiangxi Maternal & Child Hlth Hosp, Dept Pediat Surg, Nanchang 330100, Jiangxi, Peoples R China; [Zeng, Qiang; Liu, Tingting; Tao, Junfeng] Chongqing Med Univ, Dept Pediat Surg, Jiangxi Hosp, Childrens Hosp, Nanchang 330100, Jiangxi, Peoples R China

通信作者：Tao, JF (通讯作者)，Jiangxi Maternal & Child Hlth Hosp, Dept Pediat Surg, Nanchang 330100, Jiangxi, Peoples R China.; Tao, JF (通讯作者)，Chongqing Med Univ, Dept Pediat Surg, Jiangxi Hosp, Childrens Hosp, Nanchang 330100, Jiangxi, Peoples R China.

来源：BMC CANCER

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001258129500003

JCR分区：Q2

影响因子：3.4

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Wilms tumor; Molecular biomarkers; EMCN; CCNA1

摘要：BackgroundWilms tumor (WT) is the most common pediatric embryonal tumor. Improving patient outcomes requires advances in understanding and targeting the multiple genes and cellular control pathways, but its pathogenesis is currently not well-researched. We aimed to identify the potential molecular biological mechanism of WT and develop new prognostic markers and molecular targets by comparing gene expression profiles of Wilms tumors and fetal normal kidneys.MethodsDifferential gene expression analysis was performed on Wilms tumor transcriptomic data from the GEO and TARGET databases. For biological functional analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were utilized. Out of 24 hub genes identified, nine were found to be prognostic-related through univariate Cox regression analysis. These nine genes underwent LASSO regression analysis to enhance the predictive capability of the model. The key hub genes were validated in the GSE73209 datasets, and cell function experiments were conducted to identify the genes' functions in WiT-49 cells.ResultsThe enrichment analysis revealed that DEGs were significantly involved in the regulation of angiogenesis and regulation of cell differentiation. 24 DEGs were identified through PPI networks and the MCODE algorithm, and 9 of 24 genes were related to WT patients' prognosis. EMCN and CCNA1 were identified as key hub genes, and related to the progression of WT. Functionally, over-expression of EMCN and CCNA1 knockdown inhibited cell viability, proliferation, migration, and invasion of Wilms tumor cells.ConclusionsEMCN and CCNA1 were identified as key prognostic markers in Wilms tumor, suggesting their potential as therapeutic targets. Differential gene expression and enrichment analyses indicate significant roles in angiogenesis and cell differentiation.

基金机构：Science and Technology Research Project of Jiangxi Provincial Department of Education [GJJ2203544]

基金资助正文：Science and Technology Research Project of Jiangxi Provincial Department of Education, GJJ2203544.