Variation in Chongqing finger citron components and antioxidant activity from essential oils in several organs

作者全名："Fang-Hong, Shang; Yu, Chen; Hong-Fei, Du; Jiang, He; Zhen, Wu; Xu-Hong, Song; Long-Yun, Li"

作者地址："[Fang-Hong, Shang; Yu, Chen; Hong-Fei, Du; Jiang, He; Zhen, Wu; Xu-Hong, Song; Long-Yun, Li] Chongqing Acad Chinese Mat Med, Chongqing 400065, Peoples R China; [Fang-Hong, Shang] Sichuan Chongqing Joint Key Lab creat New Chinese, Chongqing 400065, Peoples R China; [Yu, Chen; Jiang, He] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China; [Xu-Hong, Song; Long-Yun, Li] China Acad Chinese Med Sci, Chongqing Sub Ctr Natl Resources, Ctr Chinese Mat Med, Chongqing 400065, Peoples R China"

通信作者："Fang-Hong, S (通讯作者)，Chongqing Acad Chinese Mat Med, Chongqing 400065, Peoples R China."

来源：SOUTH AFRICAN JOURNAL OF BOTANY

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001262827400001

JCR分区：Q2

影响因子：3.1

年份：2024

卷号：171

期号：　

开始页：504

结束页：516

文献类型：Article

关键词：Finger citron; Chongqing; Essential oils; Organs; Gas chromatography-mass spectrometry; Antioxidants

摘要："The finger citron (Citrus medica L.var. sarcodactylis Swingle) is a both medicinal and edible species specified by the China Ministry of Health, It is also a regional medicinal plant grown in China's Chongqing Municipality. We explored the utilization value of flower, leaf, and branch resources of non-medicinal sections of finger citron of Chongqing origin and identified variations in its yield, content, and antioxidant activity in essential oils in several organs. From several finger citron organs, essential oils were extracted using ultrasonic-assisted steam distillation, and their essential components were identified using gas chromatography-mass spectrometry (GC-MS). To assess antioxidant properties of various finger citron organs, DPPH free radical scavenging rate, ABTS free radical scavenging rate, and total reducing power of essential oils were determined. A total of 71 chemical components were discovered, and a cluster heat map revealed that d-limonene, Citral, (Z)-3,7-dimethylocta-2,6-dienal, gamma-Terpinene, OCymene, and Ocimene were the most significant components and that their composition and concentration in essential oils of different organs varied substantially, with the average total amount in the four major organs in descending order: branches (79.34 %), leaves (73.40 %), flowers (68.85 %), and dried fruits (55.69 %). Finger citron's non-medicinal sections and organs' essential oils exhibited some level of antioxidant activity. The maximal clearance rate of the final flower group was 19.26 % when the essential oil concentration of each group was 4.0 mg center dot mL(-1) for the assessment of DPPH free radical scavenging activity. DPPH free radical clearance rates of the bud group, initial flower group, full flower group, final flower group, branch group, and young leaf group were significantly different from the medicinal parts dried fruits group (P < 0.05), whereas there was no significant difference in the old leaf group (P > 0.05). When the essential oil concentration of each group was 0.10 mg center dot mL(-1) for the ABTS radical scavenging ability measurement, the bud group had the highest scavenging rate of 32.24 %, and significant differences in the ABTS radical scavenging rates of the bud group, primary flower group, full flower group, final flower group, and branch group were observed when compared to the dried fruits group (P < 0.05). Between the young leaf and old leaf groups, there was no significant difference (P > 0.05). When the essential oil concentration was 3.0 mg center dot mL(-1) for the measurement of overall reduction capacity, the old leaf group's highest absorbance value was 0.310. and the total reduction capacity of the bud group, first flower group, complete flower group, last flower group, branch group, young leaf group, and old leaf group substantially differed from the dried fruits (P < 0.05). This study provides a reference for the formation of ""green"" concurrent consumption and development of medicinal and non-medicinal elements of Chinese medicinal materials as well as theoretical support for the diverse utilization of finger citron resources. (c) 2024 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies."

基金机构："Fundamental Research Funds of Chongqing [cstc2021jxjl-jbky10004]; Agriculture Research System of China [CARS-21]; Chongqing Science and Technology Bureau [cstc2021jscx-dxwtBX0011, CSTB2022TIAD-KPX0094]"

基金资助正文："This work was supported by Fundamental Research Funds of Chongqing (Grant No. cstc2021jxjl-jbky10004) , Agriculture Research System of China (CARS-21) , and the Chongqing Science and Technology Bureau (cstc2021jscx-dxwtBX0011, and CSTB2022TIAD-KPX0094) . The authors also thank Chen Guo from Diwang Agriculture Co., Ltd., Tongnan District, Chongqing for Citrus medica L. var. sarco-dactylis resources and helpful suggestions. The authors would like to express their gratitude to EditSprings (https:// www.editsprings.cn) for the expert linguistic services provided."