"Expression and Clinical Significance of NUDCD1, PI3K/AKT/mTOR Signaling Pathway-Related Molecules and Immune Infiltration in Breast Cancer"

作者全名："Wen, Wei; Li, Yuehua; Cao, Xi; Li, Yanyan; Liu, Ziyi; Tang, Zhuoqi; Xie, Liming; He, Rongfang"

作者地址："[Wen, Wei; Cao, Xi; Li, Yanyan; Liu, Ziyi; Tang, Zhuoqi; He, Rongfang] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pathol, Hengyang 421001, Hunan, Peoples R China; [Wen, Wei] Chongqing Med Univ, Yongchuan Hosp, Dept Orthoped, Chongqing 402160, Peoples R China; [Li, Yuehua; Xie, Liming] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Oncol, Hengyang 421001, Hunan, Peoples R China"

通信作者："He, RF (通讯作者)，Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pathol, Hengyang 421001, Hunan, Peoples R China.; Xie, LM (通讯作者)，Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Oncol, Hengyang, Hunan, Peoples R China."

来源：CLINICAL BREAST CANCER

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001281282700001

JCR分区：Q3

影响因子：3.1

年份：2024

卷号：24

期号：6

开始页：e429

结束页：e451

文献类型：Article

关键词：Breast cancer; NUDCD1; PI3K/AKT/mTOR pathway; Immune infiltration; Bioinformatics analysis

摘要："Breast cancer is one of the most common malignant tumors in women. In this study, we analyzed 1226 and 214 breast cancer cases using bioinformatics methods and immunohistochemical experiments, respectively. NUDCD1 was found to be highly expressed in breast cancer and may be associated with poor clinical prognosis. It may be a new therapeutic target for breast cancer patients. Background: NUDCD1 (NudC Domain Containing 1) performs an essential function in biological processes such as cell progression, migration, cell cycle, and intracellular material transport. Many solid tumors express it highly, which is a prospective biomarker and therapeutic approach. However, the expression and clinical importance of NUDCD1 across breast cancer is unclear. Methods: The expressions of NUDCD1 in breast cancers and normal breast tissues were studied utilizing the TIMER database and immunohistochemical analysis. Subsequently, we validate the association between the expression of NUDCD1 and clinicopathologic features and prognosis of breast cancer. The immunohistochemical experiments of pathway-related molecules were done on 214 breast cancer tissue microarrays. The investigation of correlation between NUDCD1 expression and tumor immune infiltration was subsequently conducted. Results: Through the utilization of bioinformatics analysis and immunohistochemical experiments, it was determined that NUDCD1 exhibited upregulation within breast cancer. Furthermore, it was discovered that an elevated expression of NUDCD1 may potentially be linked to a worse prognosis in breast cancer. Our study reveals that the PI3K/AKT/mTOR signaling pathway may perform a function in NUDCD1 regulating breast cancer progression via enrichment analysis. Furthermore, the expression of NUDCD1 may be associated with the degree of immunological infiltration. Conclusion: The expression of NUDCD1 was explored to be elevated in breast cancer and was observed to be correlated with a poorer prognosis. p-AKT, PI3K, AKT, mTOR, and p-mTOR expression levels underwent significant elevation in breast cancer. The function of NUDCD1 within breast cancer might be associated with the PI3K/AKT/mTOR signaling pathway."

基金机构："Health Committee of Hunan Province [20201974]; Science and Technology Department of Hunan Province , China [2021SK51818]; Natural Science Foundation of Hunan Province [2019JJ80036]"

基金资助正文："<B>Funding</B> This investigation has received financial support from the Health Committee of Hunan Province (Grant No. 20201974) , the Science and Technology Department of Hunan Province , China (Grant No.2021SK51818) and the Natural Science Foundation of Hunan Province (Grant No. 2019JJ80036) ."