Polyvinylpyrrolidone-curcumin nanoparticles with immune regulatory and metabolism regulatory effects for the treatment of experimental autoimmune uveitis
作者全名："Cao, Fan; Liang, Kun; Tang, Wei-Wei; Ni, Qin-Yu; Ji, Zhi-Yu; Zha, Chen-Kai; Wang, Ya-Kun; Jiang, Zheng-Xuan; Hou, Shengping; Tao, Li-Ming; Wang, Xianwen"
作者地址："[Cao, Fan; Liang, Kun; Tang, Wei-Wei; Ni, Qin-Yu; Ji, Zhi-Yu; Jiang, Zheng-Xuan; Tao, Li-Ming] Anhui Med Univ, Dept Ophthalmol, Affiliated Hosp 2, Hefei 230601, Peoples R China; [Cao, Fan; Ni, Qin-Yu; Ji, Zhi-Yu; Zha, Chen-Kai] Anhui Med Univ, Sch Clin Med 2, Dept Clin Med, Hefei 230032, Peoples R China; [Wang, Ya-Kun] Chongqing Med Univ, Dept Ophthalmol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Hou, Shengping] Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China; [Wang, Xianwen] Anhui Med Univ, Affiliated Hosp 2, Res Ctr Translat Med, Hefei 230601, Peoples R China; [Wang, Xianwen] Anhui Med Univ, Res & Engn Ctr Biomed Mat, Sch Biomed Engn, Hefei 230032, Peoples R China"
通信作者："Jiang, ZX; Tao, LM (通讯作者)，Anhui Med Univ, Dept Ophthalmol, Affiliated Hosp 2, Hefei 230601, Peoples R China.; Hou, SP (通讯作者)，Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China.; Wang, XW (通讯作者)，Anhui Med Univ, Affiliated Hosp 2, Res Ctr Translat Med, Hefei 230601, Peoples R China."
来源：JOURNAL OF CONTROLLED RELEASE
ESI学科分类：PHARMACOLOGY & TOXICOLOGY
WOS号：WOS:001282473900001
JCR分区：Q1
影响因子：10.8
年份：2024
卷号：372
期号：
开始页：551
结束页：570
文献类型：Article
关键词：Nanoparticles; Curcumin; Polyvinylpyrrolidone; Experimental autoimmune uveitis; EAU; Inflammation
摘要："Uveitis comprises a cluster of intraocular inflammatory disorders characterized by uncontrolled autoimmune responses and excessive oxidative stress leading to vision loss worldwide. In the present study, curcumin (CUR) was conjugated with polyvinylpyrrolidone (PVP) to form PVP-CUR nanoparticles with significantly elevated solubility and outstanding multiple radical scavenging abilities. In vitro studies revealed that PVP-CUR nano- particles markedly mitigated oxidative stress and reduced apoptosis in a H2O2-induced 2 O 2-induced human retinal pigment epithelial cell line (ARPE-19) and promoted phenotypic polarization from M1 to M2 in an LPS-induced human microglial cell line (HMC3). Further in vivo studies demonstrated the prominent therapeutic effects of PVP-CUR nanoparticles on experimental autoimmune uveitis (EAU), which relieved clinical and pathological progression, improved perfusion and tomographic manifestations of retinal vessels, and reduced blood-retinal barrier (BRB) leakage; these effects may be mediated by mitigating oxidative stress and attenuating macrophage/microgliaelicited inflammation. Notably, treatment with PVP-CUR nanoparticles was shown to regulate metabolite alterations in EAU rats, providing novel insights into the underlying mechanisms involved. Additionally, the PVPCUR nanoparticles showed great biocompatibility in vivo. . In summary, our study revealed that PVP-CUR nanoparticles may serve as effective and safe nanodrugs for treating uveitis and other oxidative stress- and inflammation-related diseases."
基金机构："National Natural Science Foundation of China [82070986, 82171043, 82371080]; Scientific Research Project of Colleges and Universities in Anhui Province [2023AH053179]; Natural Science Funds for Distinguished Young Scholar of Anhui Province [2308085J29, 2023AH020046]; Excellent Youth of Natural Science Research Projects in Anhui Province Univer-sities [2023AH030060]; Anhui Medical University Fund [2021xkj161]; Basic and Clinical Cooperative Research and Promotion Program of Anhui Medical University [2021xkjT028]"
基金资助正文："This work was supported by the National Natural Science Foundation of China (82070986, 82171043, 82371080) , the Scientific Research Project of Colleges and Universities in Anhui Province (2023AH053179) , the Natural Science Funds for Distinguished Young Scholar of Anhui Province (2308085J29, 2023AH020046) , the Excellent Youth of Natural Science Research Projects in Anhui Province Univer-sities (2023AH030060) , the Anhui Medical University Fund funded project (2021xkj161) , and the Basic and Clinical Cooperative Research and Promotion Program of Anhui Medical University (2021xkjT028) . The authors would like to thank the Shiyan laboratory ( www.shiyanjia.com ) for their help in language polishing."
