Human amniotic epithelial cells improve uterine spiral artery remodeling to ameliorate preeclampsia in a rat model

作者全名："Geng, Lanxin; Qin, Zuchao; Han, Ting-Li; Zhou, Yanqiu; Zhong, Xiaocui; Zhang, Guanghui; Dong, Xiaojing"

作者地址："[Geng, Lanxin; Qin, Zuchao; Han, Ting-Li; Zhou, Yanqiu; Zhong, Xiaocui; Dong, Xiaojing] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, 76,Linjiang Rd, Chongqing 400010, Peoples R China; [Zhang, Guanghui] Chongqing Saiyao Biotechnol Co Ltd, Chongqing, Peoples R China"

通信作者："Dong, XJ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, 76,Linjiang Rd, Chongqing 400010, Peoples R China."

来源：BIOLOGY OF REPRODUCTION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001283462100001

JCR分区：Q2

影响因子：3.6

年份：2024

卷号：111

期号：4

开始页：906

结束页：918

文献类型：Article

关键词：human amniotic epithelial cells (hAECs); preeclampsia; spiral artery remodeling; inflammatory; offspring neurological development

摘要："Preeclampsia (PE) is a multisystem pregnancy disorder characterized by impaired remodeling of placental spiral arteries, which leads to the release of pro-inflammatory cytokines and anti-angiogenic agents. However, treatment options for PE are limited, with termination of pregnancy being the only curative option. In this work, we investigated the effects of human amniotic epithelial cells (hAECs) in PE rat model. The rats were induced with lipopolysaccharide (LPS) on gestational day 14.5 followed by injection of hAECs and human umbilical cord mesenchymal stem cells 24 h later. The hAECs treatment resulted in a reduction in blood pressure and proteinuria in the PE rat model. Furthermore, hAECs treatment decreased levels of pro-inflammatory cytokines, reduced inflammatory cells aggregation, and alleviated the damage to placental spiral arteries by downregulating the expression of anti-angiogenic factor and upregulating proangiogenic factor. In vitro experiments confirmed that hAECs treatment restored the proliferation, migration, and angiogenesis of LPS-damaged human umbilical vein endothelial cells. Additionally, hAECs treatment had positive effects on fetal weight and neurological development in the PE group, with no negative effects on the physical development or fertility of offspring rats. These results suggested that hAECs transplantation may be a novel adjuvant therapeutic strategy for PE by reducing the inflammatory and enhancing placental spiral artery angiogenesis."

基金机构："Chongqing Municipal Education Commission [KJZD-M202300401]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0122]; National Clinical Study of Child Health and Disease [NCRCCHD-2020-GP-16]; Kuanren Talents Programs of the Second Affiliated Hospital of Chongqing Medical University"

基金资助正文："This work was supported by Chongqing Municipal Education Commission (KJZD-M202300401), Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0122), National Clinical Study of Child Health and Disease (NCRCCHD-2020-GP-16), and Kuanren Talents Programs of the Second Affiliated Hospital of Chongqing Medical University."