Exploring the therapeutic potential of urine-derived stem cell exosomes in temporomandibular joint osteoarthritis

作者全名："Zhou, Jian-Ping; Peng, Si-Si; Xu, Jie; Cheng, Xing-Wang; Wang, Xiao-Hui; Tao, Jun-Li; Dai, Hong-Wei; Cao, Xin"

作者地址："[Zhou, Jian-Ping; Peng, Si-Si; Xu, Jie; Wang, Xiao-Hui; Tao, Jun-Li; Dai, Hong-Wei; Cao, Xin] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; [Zhou, Jian-Ping; Peng, Si-Si; Xu, Jie; Wang, Xiao-Hui; Tao, Jun-Li; Dai, Hong-Wei; Cao, Xin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Zhou, Jian-Ping; Peng, Si-Si; Xu, Jie; Wang, Xiao-Hui; Tao, Jun-Li; Dai, Hong-Wei; Cao, Xin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Cheng, Xing-Wang] Chongqing Med Univ, Ctr Joint Surg, Dept Orthoped Surg, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Dai, HW; Cao, X (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：FASEB JOURNAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001283549400001

JCR分区：Q1

影响因子：4.8

年份：2024

卷号：38

期号：15

开始页：　

结束页：　

文献类型：Article

关键词：exosomes; mechanical force; proliferation; TMJOA; USC

摘要："Temporomandibular joint osteoarthritis (TMJOA) is a degenerative ailment that causes slow cartilage degeneration, aberrant bone remodeling, and persistent discomfort, leading to a considerable reduction in the patient's life quality. Current treatment options for TMJOA have limited efficacy. This investigation aimed to explore a potential strategy for halting or reversing the progression of TMJOA through the utilization of exosomes (EXOs) derived from urine-derived stem cells (USCs). The USC-EXOs were obtained through microfiltration and ultrafiltration techniques, followed by their characterization using particle size analysis, electron microscopy, and immunoblotting. Subsequently, an in vivo model of TMJOA induced by mechanical force was established. To assess the changes in the cartilage of TMJOA treated with USC-EXOs, we performed histology analysis using hematoxylin-eosin staining, immunohistochemistry, and histological scoring. Our findings indicate that the utilization of USC-EXOs yields substantial reductions in TMJOA, while concurrently enhancing the structural integrity and smoothness of the compromised condylar cartilage surface. Additionally, USC-EXOs exhibit inhibitory effects on osteoclastogenic activity within the subchondral bone layer of the condylar cartilage, as well as attenuated apoptosis in the rat TMJ in response to mechanical injury. In conclusion, USC-EXOs hold considerable promise as a potential therapeutic intervention for TMJOA. In this investigation, the use of human urine-derived stem cell exosomes (USC-EXOs) prevented or reversed the progression of mechanical force-induced temporomandibular joint osteoarthritis (TMJOA). USC-EXOs significantly inhibited chondrocyte apoptosis and release of inflammatory factors and promoted chondrocyte proliferation, thereby increasing the structural integrity and smoothness of the cartilage surfaces of damaged condyles. USC-EXO has a promising future as a potential therapeutic intervention for TMJ injuries.image"

基金机构：CSTC | Natural Science Foundation of Chongqing Municipality [CSTB2022NSCQ-MSX0982]; Open Project of State Key Laboratory of Ultrasonic Medical Engineering [2022KFKT003]

基金资助正文："CSTC | Natural Science Foundation of Chongqing Municipality, Grant/Award Number: CSTB2022NSCQ- MSX0982; Open Project of State Key Laboratory of Ultrasonic Medical Engineering, Grant/Award Number: 2022KFKT003"