Leptin protects chondrocytes by inhibiting autophagy via phosphoinositide 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway

作者全名："Li, Ping; Jiang, Weiqian; Yang, Qiming; Lu, Yang; Zhang, Jian"

作者地址："[Li, Ping; Lu, Yang; Zhang, Jian] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthopaed, 1 You Yi Rd, Chongqing 400016, Peoples R China; [Li, Ping; Yang, Qiming] Chongqing Hosp Tradit Chinese Med, Dept Orthopaed, Chongqing 400021, Peoples R China; [Jiang, Weiqian] Chongqing Med Univ, Affiliated Hosp 2, Dept Orthopaed, Chongqing 400016, Peoples R China"

通信作者："Zhang, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthopaed, 1 You Yi Rd, Chongqing 400016, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001291414800001

JCR分区：Q2

影响因子：4

年份：2024

卷号：10

期号：15

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Leptin has been widely studied and found to have a significant impact on the development of osteoarthritis (OA). However, there are conflicting findings regarding the impact of leptin on chondrocytes. The study aimed to examine the impact of leptin on human chondrocytes and rats with OA. In the in vitro experiment, cartilage tissue obtained from patients hospitalized for knee replacement due to OA was collected for primary culture of chondrocytes. The proliferation and apoptosis of chondrocytes were assessed using cell counting kit-8 and flow cytometry. Autophagy levels were evaluated through monodansylcadaverine staining, mRFP-GFP-LC3 fluorescence, and transmission electron microscopy. Additionally, the expression of autophagy-related genes and proteins was analyzed using qRT-PCR and western blotting. In the in vivo experiment, an OA rat model was established. Following treatment with leptin and leptin antagonists, the cartilage tissues were examined using histology analysis (hematoxylin-eosin and Safranin O/fast green staining) and immunohistochemical. Mankin's score was utilized to assess the severity of OA, while qRT-PCR and western blotting were employed to detect the expression of autophagy-related genes and proteins in the cartilage. The ability of leptin to protect chondrocytes is achieved through the inhibition of autophagy via phosphoinositide 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway."

基金机构：Medical research project of Chongqing Talents Program [CQYC202010]

基金资助正文：Medical research project of Chongqing Talents Program (Grant No. CQYC202010) .