TROP2 is highly expressed in triple-negative breast cancer CTCs and is a potential marker for epithelial mesenchymal CTCs

作者全名："Liao, Qingyu; Zhang, Ruiming; Ou, Zuli; Ye, Yan; Zeng, Qian; Wang, Yange; Wang, Anqi; Chen, Tingmei; Chai, Chengsen; Guo, Bianqin"

作者地址："[Liao, Qingyu; Zhang, Ruiming; Ou, Zuli; Ye, Yan; Zeng, Qian; Wang, Yange; Chen, Tingmei; Chai, Chengsen; Guo, Bianqin] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Guo, Bianqin] Chongqing Univ, Dept Clin Lab, Canc Hosp, Chongqing 40030, Peoples R China; [Wang, Anqi] Chinese Acad Sci, Natl Ctr Nanosci & Technol, Key Lab Biomed Effects Nanomat & Nanosafety, Key Lab Standardizat & Measurement Nanotechnol,Ctr, Beijing 100190, Peoples R China"

通信作者："Chai, CS; Guo, BQ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：MOLECULAR THERAPY ONCOLOGY

ESI学科分类：　

WOS号：WOS:001295178500001

JCR分区：　

影响因子：　

年份：2024

卷号：32

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Circulating tumor cells (CTCs) are the seeds of distant metastases of malignant tumors and are associated with malignancy and risk of metastasis. However, tumor cells undergo epithelial-mesenchymal transition (EMT) during metastasis, leading to the emergence of different types of CTCs. Real-time dynamic molecular and functional typing of CTCs is necessary to precisely guide personalized treatment. Most CTC detection systems are based on epithelial markers that may fail to detect EMT CTCs. Therefore, it is clinically important to identify new markers of different CTC types. In this study, bioinformatics analysis and experimental assays showed that trophoblast cell surface antigen 2 (TROP2), a target molecule for advanced palliative treatment of triple-negative breast cancer (TNBC), was highly expressed in TNBC tissues and tumor cells. Furthermore, TROP2 can promote the migration and invasion of TNBC cells by upregulating EMT markers. The specificity and potential of TROP2 as an EMT-associated marker of TNBC CTCs were evaluated by flow cytometry, immunofluorescence, spiking experiments, and a well-established CTC assay. The results indicated that TROP2 is a potential novel CTC marker associated with EMT, providing a basis for more efficacious markers that encompass CTC heterogeneity in patients with TNBC."

基金机构：Foundation of Chongqing Municipal Education Commission [HZ2021006]; Natural Science Foundation of Chongqing [CSTB2023NSCQ-MSX0845]

基金资助正文："This work was supported by the Foundation of Chongqing Municipal Education Commission (grant no. HZ2021006) , the Natural Science Foundation of Chongqing (grant no. CSTB2023NSCQ-MSX0845"