Study on The Promotion of Tenocyte Proliferation and Differentiation by Oriented Fiber Membrane Loaded With Nano-zinc Oxide

作者全名："Fan, Jia; Gu, Peng-Cheng; Cheng, Xi-Ting; Jiang, Qiang; Zhao, Ya; Pan, Xiao-Fang; Bai, Yan"

作者地址："[Fan, Jia; Gu, Peng-Cheng; Cheng, Xi-Ting; Jiang, Qiang; Zhao, Ya; Pan, Xiao-Fang; Bai, Yan] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China"

通信作者："Bai, Y (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China."

来源：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001303636600013

JCR分区：Q4

影响因子：0.3

年份：2024

卷号：51

期号：8

开始页：1895

结束页：1903

文献类型：Article

关键词：nano zinc oxide; oriented fibers; tendon repair

摘要："Objective To simulate the microstructure and mechanical properties of tendon tissue and promote its regeneration and repair, electrospinning technology was used to prepare L-polylactic acid (PLLA) fiber membranes loaded with different nano zinc oxide contents and with oriented structures. Physical and chemical characterization and biological performance evaluation were carried out to explore their effects on tendon cell proliferation and differentiation. Methods Preparation of PLLA fiber scaffolds and PLLA/ZnO fiber scaffolds containing different mass fractions of nano ZnO was performed using electrospinning technology. The physicochemical properties of the scaffold were characterized by scanning electron microscopy, mechanical stretching, and EDS spectroscopy. The scaffold was co-cultured with mouse tendon cells to detect its biocompatibility and regulatory effects on cell differentiation behavior. Results The fiber scaffolds were arranged in an oriented manner, and zinc elements were uniformly distributed in the fibers. The tensile strength and Young's modulus of PLLA/0.1%ZnO fiber scaffolds were significantly higher than PLLA fiber scaffolds. The number of cells on the surface of PLLA/0.1%ZnO fiber scaffold was significantly higher than that of the PLLA group, and the activity was better; mouse tendon cells exhibit directional adhesion and growth along the fiber arrangement direction. Conclusion The oriented PLLA/0.1%ZnO fiber scaffold had excellent physicochemical properties, which can significantly promote the oriented growth, proliferation and differentiation of tendon cells. It is expected to be used for tendon tissue regeneration and repair in the future."

基金机构：National Natural Science Foundation of China [32371415]; Chongqing Natural Science Foundation [CSTB2023NSCQ-MSX0307]; Development Support Program for Future Medical Youth Innovation Team of Chongqing Medical University [W0055]

基金资助正文："This work was supported by grants from The National Natural Science Foundation of China (32371415) , Chongqing Natural Science Foundation (CSTB2023NSCQ-MSX0307) , and Development Support Program for Future Medical Youth Innovation Team of Chongqing Medical University (W0055) ."