Bioinformatic analysis reveals the relationship between macrophage infiltration and Cybb downregulation in hyperoxia-induced bronchopulmonary dysplasia

作者全名："He, Yi; Li, Decai; Zhang, Meiyu; Li, Fang"

作者地址："[He, Yi; Li, Decai; Li, Fang] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Pediat, Women & Childrens Hosp, Chongqing 401147, Peoples R China; [He, Yi; Li, Decai; Li, Fang] Chongqing Res Ctr Prevent & Control Maternal & Chi, Chongqing 401147, Peoples R China; [Zhang, Meiyu] Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat,Dept Neonatal Diag,Childr, Chongqing 400015, Peoples R China; [Zhang, Meiyu] Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat,Treatment Ctr,Childrens H, Chongqing 400015, Peoples R China"

通信作者："Li, F (通讯作者)，Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Pediat, Women & Childrens Hosp, Chongqing 401147, Peoples R China.; Li, F (通讯作者)，Chongqing Res Ctr Prevent & Control Maternal & Chi, Chongqing 401147, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001304109300025

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Bronchopulmonary dysplasia; Macrophage; Cybb; Immune infiltration; Cell-cell crosstalk

摘要："Bronchopulmonary dysplasia (BPD) is the most common sequela of prematurity and is characterized by alveolar simplification and lung angiogenesis failure. The aim of this study was to explore the immune signatures of BPD. Differentially expressed gene analysis and immune infiltration analysis were conducted to identify key immune cell types and related genes by using the mRNA-seq dataset GSE25286. The expression patterns of key genes were validated in the scRNA-seq dataset GSE209664 and in experiments. The cell-cell crosstalk of key immune cells was explored with CellChat. We found that differentially expressed genes between BPD mice and controls were mostly enriched in leukocyte migration and M1 macrophages were highly enriched in BPD lungs. Hub genes (Cybb, Papss2, F7 and Fpr2) were validated at the single-cell level, among which the downregulation of Cybb was most closely related to macrophage infiltration. The reduced mRNA and protein levels of Cybb were further validated in animal experiments. Colocalization analysis of Cybb and macrophage markers demonstrated a significant decrease of Cybb in M1 macrophages. Cell-cell crosstalk found that alveolar epithelial cells interacted actively with macrophages through MIF-(CD74 + CD44) signalling. In conclusion, M1 macrophages played important roles in promoting BPD-like lung injury, which was correlated with a specific reduction of Cybb in macrophages and the potential activation of MIF signalling."

基金机构：National Key Research and Development Program of China

基金资助正文：No Statement Available