TYROBP serve as potential immune-related signature genes in the acute phase of intracerebral hemorrhage

作者全名："Zhang, Mi; Duan, Yuhao; Gan, Hui; Jiang, Ning; Qin, Le; Luo, Yujia; Palahati, Ailiyaer; He, Yaying; Li, Chenyang; Zhai, Xuan"

作者地址："[Zhang, Mi; Duan, Yuhao; Gan, Hui; Qin, Le; Palahati, Ailiyaer; He, Yaying; Li, Chenyang; Zhai, Xuan] Chongqing Med Univ, Dept Neurosurg, Key Lab Child Dev & Disorders,Natl Clin Res Ctr Ch, Chongqing Key Lab Child Neurodev & Cognit Disorder, Chongqing 401122, Peoples R China; [Zhang, Mi; Duan, Yuhao; Gan, Hui; Qin, Le; Palahati, Ailiyaer; He, Yaying; Li, Chenyang] Chongqing Med Univ, Sch Basic Med, Inst Neurosci, Chongqing 400016, Peoples R China; [Jiang, Ning] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Luo, Yujia] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Zhai, X (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Key Lab Child Dev & Disorders,Natl Clin Res Ctr Ch, Chongqing Key Lab Child Neurodev & Cognit Disorder, Chongqing 401122, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001304147400044

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Intracerebral hemorrhage; Signature gene; mRNA sequencing; Tyrobp

摘要："The development of intracerebral hemorrhage (ICH) is a dynamic process and intervention during the acute phase of ICH is critical for subsequent recovery. Therefore, it is crucial to screen potential signature genes and therapeutic target genes in the acute phase of ICH. In this study, based on the results of mRNA sequencing in mouse ICH and mRNA sequencing of human ICH from online databases, top five potential signature genes after ICH, Tyrobp, Itgb2, Tlr2, Ptprc and Itgam, were screened. Quantitative PCR results showed higher mRNA expression of Tyrobp, Itgb2, Tlr2, Ptprc, and Itgam in the 1-, 3- and 5-day mouse ICH groups compared to the sham-operated group. Immune infiltration correlation analysis shows that the top-ranked signature gene, Tyrobp, is negatively correlated with M2 macrophages and plasma cells, and Western blot analysis shows higher expression of the Tyrobp protein in the 1-, 3-, and 5-day mouse ICH groups compared to the sham-operated group. Furthermore, immunohistochemistry revealed that TYROBP protein expression was significantly higher in human ICH tissues than in normal brain tissues. Our results suggest that Tyrobp is a signature gene in the acute phase of ICH and may be a potential target for the treatment of the acute phase of ICH."

基金机构：Chongqing Graduate Student Research Innovation Program

基金资助正文：The authors would like to thank Prof. Jingxian Wu for providing the paraffin sections for IHC assay.