Associations of co-exposure to polycyclic aromatic hydrocarbons and lead (Pb) with IGF1 methylation in peripheral blood of preschool children from an e-waste recycling area

作者全名："Huo, Xia; Xu, Xijin; Wang, Qihua; Zhang, Jian; Hylkema, Machteld N.; Zeng, Zhijun"

作者地址："[Huo, Xia; Wang, Qihua; Zhang, Jian] Jinan Univ, Sch Environm, Lab Environm Med & Dev Toxicol, Guangdong Key Lab Environm Pollut & Hlth, Guangzhou 511443, Guangdong, Peoples R China; [Zeng, Zhijun] Chongqing Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Zeng, Zhijun] Chongqing Med Univ, Res Ctr Environm & Human Hlth, Sch Publ Hlth, Chongqing 400016, Peoples R China; [Xu, Xijin] Shantou Univ, Med Coll, Lab Environm Med & Dev Toxicol, Shantou 515041, Guangdong, Peoples R China; [Xu, Xijin] Shantou Univ, Dept Cell Biol & Genet, Med Coll, Shantou 515041, Guangdong, Peoples R China; [Hylkema, Machteld N.] Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, Hanzepl 1, NL-9713 GZ Groningen, Netherlands"

通信作者："Zeng, ZJ (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：ENVIRONMENT INTERNATIONAL

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:001325711300001

JCR分区：Q1

影响因子：11.8

年份：2024

卷号：190

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Co-exposure; PAHs and Pb; IGF1 methylation; Preschool children; E -waste; Physical growth

摘要："Background: Childhood exposure to polycyclic aromatic hydrocarbons (PAHs) or lead (Pb) is associated with epigenetic modifications. However, the effects of their co-exposures on IGF1 (Insulin-like growth factor 1) methylation and the potential role in child physical growth are unclear. Methods: From our previous children study (N = 238, ages of 3-6), 75 children with higher total concentrations of urinary ten hydroxyl PAH metabolites (& sum;10OH-PAHs) from an e-waste recycling area, Guiyu, and 75 with lower & sum;10OH-PAHs from Haojiang (reference area) were included. Pb and IGF1 P2 promoter methylation in peripheral blood were also measured. Multivariable linear regression analyses were performed to estimate individual associations, overall effects and interactions of co-exposure to OH-PAHs and Pb on IGF1 methylation were further explored using Bayesian kernel machine regression. Results: Methylation of IGF1 (CG-232) was lower (38.00 vs. 39.74 %, P < 0.001), but of CG-207 and CG-137 were higher (59.94 vs. 58.41 %; 57.60 vs. 56.28 %, both P < 0.05) in exposed children than the reference. The elevated urinary 2-OHPhe was associated with reduced methylation of CG-232 (B = -0.051, 95 % CI: -0.096, -0.005, P < 0.05), whereas blood Pb was positively associated with methylation of CG-108 (B = 0.106, 95 %CI: 0.013, 0.199, P < 0.05), even after full adjustment. Methylations of CG-224 and 218 significantly decreased when all OH-PAHs and Pb mixtures were set at 35th - 40th and 45th - 55th percentile compared to when all fixed at 50th percentile. There were bivariate interactions of co-exposure to the mixtures on methylations of CG232, 224, 218, and 108. Methylations correlated with height, weight, were observed in the exposed children. Conclusions: Childhood co-exposure to high PAHs and Pb from the e-waste may be associated with IGF1 promoter methylation alterations in peripheral blood. This, in turn, may interrupt the physical growth of preschool children."

基金机构：High-level Talents Project of Chongqing Medical University [R4027]; National Natural Science Foundation of China [22376079]; National Foreign Expert Project of Chinese Ministry of Science and Technology [G2022199007L]

基金资助正文："This work was supported by the High-level Talents Project of Chongqing Medical University (R4027 to Z.J.Z) , the National Natural Science Foundation of China (22376079) and the National Foreign Expert Project of Chinese Ministry of Science and Technology (G2022199007L) . We are also grateful to all the recruited children and their guardians for participating in this project."