Highly sensitive fluorescence biosensing of BCR-ABL1 fusion gene based on exponential transcription-triggered hemin catalysis

作者全名："Kang, Lina; Duan, Jiaxin; He, Fang; Teng, Jie; Li, Jia; Yang, Tiantian; Luo, Nini; Zhao, Jie; Ding, Shijia; Cheng, Wei"

作者地址："[Kang, Lina; Duan, Jiaxin; Teng, Jie; Yang, Tiantian; Luo, Nini; Zhao, Jie; Cheng, Wei] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [He, Fang] Chongqing Med Univ, Dept Pharm, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Li, Jia; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Cheng, W (corresponding author), Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000600787800134

JCR分区：Q1

影响因子：6.1

年份：2021

卷号：224

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：BCR-ABL1 fusion gene; Chronic myeloid leukemia; Exponential transcription; Hemin-mimetic enzymes; Fluorescence biosensing

摘要："Simple, sensitive and specific detection of the transcription level of BCR-ABL1 mRNA possesses vital clinical significance in diagnosis and treatment of chronic myeloid leukemia (CML). In this study, an innovative fluorescence biosensing methodology has been developed for sensitive and specific detection of BCR-ABL1 mRNA by integrating high-efficiency of exponential transcription and superior catalytic performance of DNA-grafted hemin. Exponential transcription was triggered by BCR-ABL1 mRNA to produce plenty of RNA products. They can specifically hybridize with circular dual-labeled hemin (DLH) probe to dissociate the intramolecular hemin dimmers into highly active hemin monomers for catalyzing fluorescence substrate tyramine. This exponential transcription-triggered hemin catalysis (ET-HC) strategy showed highly sensitive and specific for BCR-ABL1 detection with a limit of detection at 0.5 aM and a good linear range from 2 aM to 200 fM. This method was successfully applied to directly detect as low as 0.001% e13a2 transcript isoforms from complex genomic RNA extraction. Compared with clinical routine, the overall process is a thermostatic reaction and eliminates additional reverse transcription operation. Therefore, the developed ET-HC strategy might provide a promising alternative tool for precise diagnosis and personalized treatment of CML."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81873972, 81873980]; Science Fund of Chongqing for Distinguished Young Scholars [cstc2019jcyjjqX0028]; Training Program for Advanced Young Medical Personnel of Chongqing [2017HBRC003]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (81873972 and 81873980), the Science Fund of Chongqing for Distinguished Young Scholars (cstc2019jcyjjqX0028), the Training Program for Advanced Young Medical Personnel of Chongqing (2017HBRC003)."