Leptin correlates with monocytes activation and severe condition in COVID-19 patients

作者全名："Wang, Jingya; Xu, Yinyin; Zhang, Xiaoli; Wang, Sheng; Peng, Ziyi; Guo, Jing; Jiang, Hongmei; Liu, Jing; Xie, Ying; Wang, Jingjing; Li, Xin; Liao, Juan; Wan, Chao; Yu, Lihua; Hu, Jinsong; Liu, Beizhong; Liu, Zhiqiang"

作者地址："[Wang, Jingya; Wang, Sheng; Peng, Ziyi; Guo, Jing; Jiang, Hongmei; Liu, Jing; Xie, Ying; Wang, Jingjing; Li, Xin; Liu, Zhiqiang] Tianjin Med Univ, Sch Basic Med Sci, Tianjin Key Lab Cellular Homeostasis & Human Dis, Tianjin, Peoples R China; [Wang, Jingya; Wang, Sheng; Peng, Ziyi; Guo, Jing; Jiang, Hongmei; Liu, Jing; Xie, Ying; Wang, Jingjing; Li, Xin; Liu, Zhiqiang] Tianjin Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Tianjin 300070, Peoples R China; [Xu, Yinyin; Zhang, Xiaoli; Liao, Juan; Wan, Chao; Yu, Lihua; Liu, Beizhong] Chongqing Med Univ, Clin Lab, Yongchuan Hosp, Chongqing 402160, Peoples R China; [Xu, Yinyin; Zhang, Xiaoli; Liao, Juan; Wan, Chao; Yu, Lihua; Liu, Beizhong] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing, Peoples R China; [Hu, Jinsong] Xi An Jiao Tong Univ, Dept Cell Biol & Genet, Hlth Sci Ctr, Xian, Shaanxi, Peoples R China; [Hu, Jinsong] Xi An Jiao Tong Univ, Inst Infect & Immun, Hlth Sci Ctr, Xian, Shaanxi, Peoples R China"

通信作者："Liu, ZQ (corresponding author), Tianjin Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Tianjin 300070, Peoples R China.; Liu, BZ (corresponding author), Chongqing Med Univ, Clin Lab, Yongchuan Hosp, Chongqing 402160, Peoples R China.; Hu, JS (corresponding author), Xi An Jiao Tong Univ, Inst Infect & Immun, Hlth Sci Ctr, Dept Cell Biol & Genet, Xian 710061, Peoples R China."

来源：JOURNAL OF LEUKOCYTE BIOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000604986400001

JCR分区：Q2

影响因子：5.5

年份：2021

卷号：110

期号：1

开始页：9

结束页：20

文献类型：Article

关键词：overweight cases; cytokine storm; inflammation

摘要："Excessive monocyte activation with the development of excessive or uncontrolled release of proinflammatory cytokines often results in host tissue injury and even death in patients with pneumonia caused by the 2019 novel coronavirus. However, the changes of cytokine profiles of coronavirus disease 2019 (COVID-19) patients, as well as the underlying mechanisms that are involved, remain unknown. Using a cytokine array containing 174 inflammation-related cytokines, we found significantly altered cytokine profiles in severe COVID-19 patients compared with those in mild patients or healthy controls, and identified leptin, CXCL-10, IL-6, IL-10, IL-12, and TNF-alpha as the top differentially expressed cytokines. Notably, leptin showed high consistency with CXCL-10 and TNF-alpha in predicting disease severity, and correlated with body mass index, decreased lymphocyte counts, and disease progression. Further analysis demonstrated that monocytes in severe patients with higher leptin levels were inclined toward M1 polarization. Mechanistic studies revealed that leptin synergistically up-regulated expression levels of inflammatory cytokines and surface markers with IL-6 in monocytes through STAT3 and NF-kappa B signaling pathways. Collectively, our results suggest that overweight COVID-19 patients were prone to have higher leptin levels, which further activated monocytes, resulting in amplified or dysregulated immune responses. Taken together, our findings argue that leptin correlates severity of COVID-19 and may indicate a possible mechanism by which overweight patients have a greater tendency to develop severe conditions."

基金机构："Key Technology R&D Program for COVID-19 of Tianjin Medical University [1001/F3011002]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670201, 81870161, 81900215]; Talent Project of Tianjin Medical University [11601501/2016KJ0317]; Tianjin Medical University [1001/F3011002]; Xi'an Jiaotong University Health Science Center [QNXJTU-01]; Emergency Research Project of Novel Coronavirus Pneumonia (2019-nCoV) of Chongqing Medical University; Emergency Research Project of Novel Coronavirus Pneumonia (COVID-19) of Yongchuan Hospital affiliated to Chongqing Medical University [2020XGZX098]; Natural Science Foundation of Yongchuan District, Chongqing (Ycstc) [2020nb0201]"

基金资助正文："This work was supported by the Key Technology R&D Program for COVID-19 of Tianjin Medical University (1001/F3011002, to Z.L.), the National Natural Science Foundation of China (81670201, 81870161, to Z.L.; 81900215, to Jingya Wang), the Talent Project of Tianjin Medical University (11601501/2016KJ0317, to Z.L.), special funding for research on COVID-19 from Tianjin Medical University (1001/F3011002) and Xi'an Jiaotong University Health Science Center (QNXJTU-01), the Emergency Research Project of Novel Coronavirus Pneumonia (2019-nCoV) of Chongqing Medical University, Emergency Research Project of Novel Coronavirus Pneumonia (COVID-19) of Yongchuan Hospital affiliated to Chongqing Medical University (2020XGZX098), and the Natural Science Foundation of Yongchuan District, Chongqing (Ycstc, 2020nb0201)."