The functional landscape of Golgi membrane protein 1 (GOLM1) phosphoproteome reveal GOLM1 regulating P53 that promotes malignancy
作者全名："Song, Qi; He, Xiang; Xiong, Ying; Wang, Junlan; Zhang, Lei; Leung, Elaine Lai-Han; Li, Guoping"
作者地址："[Song, Qi; He, Xiang; Zhang, Lei; Li, Guoping] Third Peoples Hosp Chengdu, Chengdu Inst Resp Hlth, Lab Allergy & Precis Med, Chengdu, Sichuan, Peoples R China; [Song, Qi; Leung, Elaine Lai-Han; Li, Guoping] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China; [Song, Qi] Southwest Med Univ, Affiliated Hosp, Dept Cardiothorac Surg, 25 Taiping St, Luzhou, Sichuan, Peoples R China; [He, Xiang; Wang, Junlan; Zhang, Lei; Li, Guoping] Chongqing Med Univ, Natl Clin Res Ctr Resp Dis, Dept Pulm & Crit Care Med, Chengdu Peoples Hosp Branch 3,Affiliated Hosp, Chengdu, Sichuan, Peoples R China; [Xiong, Ying] Hosp Sichuan Friendships, Dept Pulm & Crit Care Med, Chengdu, Sichuan, Peoples R China"
通信作者："Li, GP (corresponding author), Third Peoples Hosp Chengdu, Chengdu Inst Resp Hlth, Lab Allergy & Precis Med, Chengdu, Sichuan, Peoples R China.; Leung, ELH; Li, GP (corresponding author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China."
来源：CELL DEATH DISCOVERY
ESI学科分类：MOLECULAR BIOLOGY & GENETICS
WOS号：WOS:000625438600002
JCR分区：Q2
影响因子：7
年份：2021
卷号：7
期号：1
开始页：
结束页：
文献类型：Article
关键词：
摘要："Golgi membrane protein 1 (GOLM1) was implicated in carcinogenesis of multiple types of cancer. However, Phosphoproteome landscapes of GOLM1 overexpression in lung cancer remain largely unknown. In this study, using data from the Cancer Genome Atlas (TCGA) and phosphoproteome, we systematically evaluated the feature of GOLM1 and studied its prognostic value in non-small cell lung cancer (NSCLC). The proliferation, migration, and invasion capacities in PC9 cell with GOLM1 overexpression were determined using Trans-well system assay. Tumor engrafts was visualized in mice models and confirmed by ex vivo. An increased expression of GOLM1 had shorter overall survival (OS) in patients with NSCLC in TCGA database. GOLM1 in single gene set enrichment analysis (GSEA) related to adherent's junction, cell cycle, and pathway in cancer. Overexpression of GOLM1 in GOLM1OE PC9 cells promoted cell proliferation, migration, and invasion. Decreased migration and invasion potential were also observed in knockdown of GOLM1 in GOLM1KD PC9 cells in migration assay. An increased expression of GOLM1 could significantly increase the growth of tumor in xenograft mice models. phosphoproteome analysis showed 239 upregulated and 331 downregulated Phosphorylated proteins in GOLM1OE PC9 cells. Overexpression of GOLM1 in GSEA was significantly related to P53 in MAPK signaling pathway. Overexpression of GOLM1enhanced the phosphorylation of P53 protein at site S315 but inhibited the formation of P53 tetramers. These results indicate that overexpression GOLM1 enhances non-small-cell carcinoma aggressiveness through inhibited the formation of P53 tetramer."
基金机构："Macao Science and Technology Development Fund [0096/2018/A3]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81970026, 82000029]; project of Chengdu high level clinical key specialty, Luzhou government [2016lzxnyd-z01]; Southwest Medical University Cooperative Project [2016lzxnyd-z01]; Science and Technology Department of Sichuan Province [2018JY0380]; Health Commission of Sichuan Province [19ZD002]; Health Commission of Chengdu [2019077, 2019078]"
基金资助正文："This work is supported by the Macao Science and Technology Development Fund (Project code: 0096/2018/A3), National Natural Science Foundation of China (81970026 and 82000029), project of Chengdu high level clinical key specialty, Luzhou government and Southwest Medical University Cooperative Project (No. 2016lzxnyd-z01), Science and Technology Department of Sichuan Province (2018JY0380), and Health Commission of Sichuan Province (19ZD002), Health Commission of Chengdu (2019077 and 2019078)."
