Attenuation of Inflammation by DJ-1 May Be a Drug Target for Cerebral Ischemia-Reperfusion Injury

作者全名："Zhao, Na; Wang, Tingting; Peng, Li; Li, Yumei; Zhao, Yong; Yu, Shanshan"

作者地址："[Zhao, Na; Wang, Tingting; Peng, Li; Li, Yumei; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Basic Med Coll, Dept Pathol, Yixueyuan Rd 1, Chongqing 400016, Peoples R China; [Zhao, Na; Wang, Tingting; Peng, Li; Li, Yumei; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Mol Med Lab, Chongqing 400016, Peoples R China; [Zhao, Na; Wang, Tingting; Peng, Li; Li, Yumei; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China; [Zhao, Na; Wang, Tingting; Peng, Li; Li, Yumei; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Key Lab Neurobiol, Chongqing 400016, Peoples R China"

通信作者："Yu, SS (corresponding author), Chongqing Med Univ, Basic Med Coll, Dept Pathol, Yixueyuan Rd 1, Chongqing 400016, Peoples R China.; Yu, SS (corresponding author), Chongqing Med Univ, Mol Med Lab, Chongqing 400016, Peoples R China.; Yu, SS (corresponding author), Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China.; Yu, SS (corresponding author), Chongqing Med Univ, Key Lab Neurobiol, Chongqing 400016, Peoples R China."

来源：NEUROCHEMICAL RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000626449700003

JCR分区：Q2

影响因子：4.4

年份：2021

卷号：46

期号：6

开始页：1470

结束页：1479

文献类型：Article

关键词：DJ-1; Adeno-associated viruses; ND-13; Ischemia– reperfusion; Neuroprotection

摘要："The pathophysiological process of cerebral apoplexy is complex, and there are currently no specific drugs for this condition. The study of effective drug targets has become a hot topic in neuroscience. Currently, adeno-associated viruses (AAVs) and polypeptides are commonly used in drug research. DJ-1 has been widely considered a neuroprotective target in recent times, but the mechanism of its neuroprotective effects is unclear. In this study, we simulated ischemic injury by establishing a middle cerebral artery occlusion reperfusion (MCAO/R) model to compare the protective effect of DJ-1 overexpression induced by DJ-1 AAV and ND-13 on cerebral ischemia-reperfusion (I/R) injury. We found that DJ-1 overexpression and ND-13 significantly reduced the neurological function scores and infarct volume and alleviated pathological damage to brain tissue. In addition, Western blotting, ELISA and immunofluorescence labeling revealed that DJ-1 overexpression and ND-13 increased the expression of the anti-inflammatory cytokines IL-10 and IL-4, and decreased the levels of the pro-inflammatory cytokines IL-1 beta and TNF-alpha. In summary, our study shows that DJ-1 overexpression and ND-13 can regulate the expression of inflammatory factors and alleviate cerebral I/R injury. Thus, DJ-1 is a possible drug target for cerebral I/R injury."

基金机构：National Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0099]

基金资助正文：The National Natural Science Foundation of Chongqing (No. cstc2020jcyj-msxmX0099).