Analysis of Chronic Mild Stress-Induced Hypothalamic Proteome: Identification of Protein Dysregulations Associated With Vulnerability and Resiliency to Depression or Anxiety

作者全名："Gong, Weibo; Liao, Wei; Fang, Chui; Liu, Yanchen; Xie, Hong; Yi, Faping; Huang, Rongzhong; Wang, Lixiang; Zhou, Jian"

作者地址："[Gong, Weibo; Liao, Wei; Liu, Yanchen; Xie, Hong; Yi, Faping; Zhou, Jian] Chongqing Med Univ, Inst Neurosci, Chongqing, Peoples R China; [Gong, Weibo; Liao, Wei; Liu, Yanchen; Yi, Faping; Zhou, Jian] Chongqing Med Univ, Basic Med Coll, Chongqing, Peoples R China; [Fang, Chui; Wang, Lixiang] Shenzhen Wininnovate Biotech Co Ltd, Shenzhen, Peoples R China; [Xie, Hong] Univ Chinese Acad Sci, Chongqing Renji Hosp, Dept Pharm, Chongqing, Peoples R China; [Huang, Rongzhong] ChuangXu Inst Life Sci, Chongqing, Peoples R China"

通信作者："Zhou, J (corresponding author), Chongqing Med Univ, Inst Neurosci, Chongqing, Peoples R China.; Zhou, J (corresponding author), Chongqing Med Univ, Basic Med Coll, Chongqing, Peoples R China.; Wang, LX (corresponding author), Shenzhen Wininnovate Biotech Co Ltd, Shenzhen, Peoples R China."

来源：FRONTIERS IN MOLECULAR NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000629157600001

JCR分区：Q2

影响因子：4.8

年份：2021

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：anxiety; chronic mild stress; depression; hypothalamus; quantitative proteomics

摘要："Chronic stress as a known risk factor leads to hyperactivity of the hypothalamus-pituitary-adrenal (HPA) axis in both depression and anxiety. However, the stress-induced dysfunction of the HPA axis in these disorders especially the common and unique molecular dysregulations have not been well-explored. Previously, we utilized a chronic mild stress (CMS) paradigm to segregate and gain depression-susceptible, anxiety-susceptible, and insusceptible groups. In this study, we continue to examine the possible protein expression alterations of the hypothalamus as the center of the HPA axis in these three groups by using a proteomic approach. Though isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative analysis, a total of 593 dysregulated proteins were identified. These were potentially associated with vulnerability and adaptability of CMS-caused depression or anxiety and therefore might become novel investigative protein targets. Further independent analysis using parallel reaction monitoring (PRM) indicated that 5, 7, and 21 dysregulated proteins were specifically associated with depression-susceptible, anxiety-susceptible, and insusceptible groups, respectively, suggesting that the same CMS differently affected the regulation system of the rat hypothalamic proteome. In summary, the current proteomic research on the hypothalamus provided insights into the specific and common molecular basis for the HPA dysfunction mechanisms that underlie resiliency and vulnerability to stress-induced depression or anxiety."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770890, 31570826]; Health and Family Planning Commission of Chongqing Nanan District [2018-03]"

基金资助正文：This work was supported by grants from the National Natural Science Foundation of China (Nos. 31770890 and 31570826) and the Health and Family Planning Commission of Chongqing Nanan District (No. 2018-03).